Factors affecting adhesion of Fibrobacter succinogenes subsp. succinogenes S85 and adherence-defective mutants to cellulose
Gong, J.; Forsberg, C.W.
Applied and Environmental Microbiology 55(12): 3039-3044
ISSN/ISBN: 0099-2240 PMID: 2619302 Accession: 007340769
Fibrobacter succinogenes subsp. succinogenes, S85, formerly Bacteroides succinogenes, adheres to crystalline cellulose present in the culture medium. When the cells are suspended in buffer, adhesion is enhanced by increasing the ionic strength. Heat, glutaraldehyde, trypsin, and pronase treatments markedly reduce the extent of adhesion. Treatment with dextrinase, modification of amino and carboxyl groups with Formalin or other chemical agents, and inclusion of either albumin (1%) or Tween 80 (0.5%) do not decrease the degree of adhension. Adherence-defective mutants isolated by their inability to bind to cellulose exhibited different growth characteristics. Class 1 mutants grew on glucose, celloboise, amorphous cellulose, and crystalline cellulose. Class 2 mutants grew on glucose and amorphous cellulose but not on cellulose or crystalline cellulose. Class 3 mutants grew on glucose and cellobiose but not on amorphous or crystalline cellulose. No substanial changes were detected in the endoglucanase, cellobiosidase, and cellobiase activities of the wild type and the mutants. These data suggest that adhension to crystalline cellulose is specific and that it involves surface proteins.