EurekaMag
+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Top 15
A new species of the genus Haemogamasus from China (Acarina: Haemogamasidae)
Reference ranges for hematologic and serum biochemical values in llamas (Lama glama)
Slime mold beetles of the genus Anisotoma (Leiodidae): classification and evolution
To be young, gifted, black and female: a meditation on the cultural politics at play in representations of Venus and Serena Williams
Anti-drool cheiloplasty: clinical results in six dogs
Mentha taxonomic status as interpreted through cytology genetics and phytochemistry
Geomorphology of Lightning Creek valley, Manning Park, southwestern British Columbia
Algunos peces del Rio Autana
Development of a generic high-yield fed batch process for large-scale GMP manufacturing of human monoclonal antibodies
Treatment of 40 cases of paralysis of common peroneal nerve with acupuncture and moxibustion
Studies into the pigments in beetroot (Beta vulgaris L. ssp. vulgaris var. rubra L.)
Principles of the organization of therapeutic and preventive care in gynecologic diseases in children and adolescents
The assessment of binge eating severity among obese persons
Sample size requirements for evaluating a conservative therapy
Intensive production systems of fig (Ficus carica L.) under greenhouse conditions
+ Most Popular
Peptic ulcer and multiple papillomas of the esophagus and pylorus in an infant
Mind as a force field: comments on a new interactionistic hypothesis
Endothelin-1 plasma concentration in children and adolescents with atherogenic risk factors
Simple and rapid extraction method of total egg lipids for determining organochlorine pesticides in the egg
Management of grasslands for milk production and preservation of grass surplus
Primary duodenal tuberculosis diagnosed by endoscopic biopsy
Hypohydration and exercise: effects of heat acclimation, gender, and environment
Apropos of a Case of Esophageal Tuberculosis
Contributions to the knowledge of the Freshwater Entomostraca of South America, as shown by artificial hatching from dried material. Part i
Nutrient inputs to the Choptank River estuary: Implications for watershed management
Toxic metabolites of Amanita pantherina, A. cothurnata, A. muscaria and other Amanita species
Basic concepts and issues: a primer on distribution and sales representative agreements in the medical device and durable medical equipment industries
Recent changes in vaccination guidelines. An update on vaccines and target population initiatives
The results of research on the antioxidant emoksipin in clinical eye diseases
An overview of selected diseases and drug needs in the llama and alpaca industries
Hyperbaric oxygen therapy (HBOT) and peridontal health
Minimal Descriptors for Drumstick (Moringa oleifera Lam.) - An Underutilized Vegetable Crop
Demembrement du genre Cicindela L. (fin). V. Faune australienne. (Et liste recapitulative des genres et sous-genres proposes pour la faune mondiale)
Pollution of ground water by nutrients and fecal coliforms from lakeshore septic tank systems
Uber die Ausbruche von Maul- und Klauenseuche in den ersten 14 Tagen nach der aktiven Immunisierung mit Vakzine nach Waldmann und Kobe - Outbreaks of foot-and-mouth disease in the first 14 days after
Patologie delle alte vie respiratorie del bambino in ambulatorio: un'esperienza italiana. Diseases of the upper respiratory tract in children in ambulatory care: an Italian experience
Fundamentals of the scoliosis problem for the general practitioner
Estimating the time and temperature relationship for causation of deep-partial thickness skin burns
Containing costs in the ED
Precipitation of whey proteins concentrated by ultrafiltration

Factors determining the specificity of signal transduction by guanine nucleotide-binding protein-coupled receptors. II. Preferential coupling of the alpha 2C-adrenergic receptor to the guanine nucleotide-binding protein, Go


Factors determining the specificity of signal transduction by guanine nucleotide-binding protein-coupled receptors. II. Preferential coupling of the alpha 2C-adrenergic receptor to the guanine nucleotide-binding protein, Go



Journal of Biological Chemistry 267(14): 9852-9857



ISSN/ISBN: 0021-9258

PMID: 1349607

Cell to cell communication by many hormones and neurotransmitters involves three major entities: receptor (R), G-protein (G), and effector molecule (E). Plasticiity in this system is conferred by the existence of each entity as isoforms or closely related subtypes that are expressed in a tissue-specific and developmentally regulated manner. Factors that determine signal specificity in this system are poorly understood. Such factors include the relative affinity and stoichiometry of R-G or G-E and the possible colocalization of R-G-E in cellular microdomains. Utilizing the .alpha.2-adrenergic receptor (.alpha.2-AR) system as a representative subfamily of this class of signal transducers, we determined the relative importance of these factors. By analysis of R-G coupling in mammalian cells cotransfected with .alpha.2-AR genes and G.alpha. cDNA, we demonstrate preferential coupling between an .alpha.2-AR subtype and Go. Our data implicate R-G affinity as an important determinant of signal transduction specificity and indicate that a critical level of Go.alpha. is required for coupling. This report indicates the utility of R-G cotransfection in mammalian cells as a "natural environment model" to characterized events occurring at the R-G and G-E interface.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 007341684

Download citation: RISBibTeXText

Related references

Factors determining the specificity of signal transduction by guanine nucleotide-binding protein-coupled receptors. II: Preferential coupling of the α2c-adrenergic receptor to the guanine nucleotide-binding protein, Go. The Journal of Biological Chemistry 267(14): 9852-9857, 1992

Factors determining the specificity of signal transduction by guanine nucleotide-binding protein-coupled receptors. II. Preferential coupling of the a2C-adrenergic receptor to the guanine nucleotide-binding protein, Go. The Journal of Biological Chemistry 267: 52-7, 1992

Factors determining the specificity of signal transduction by guanine nucleotide-binding protein-coupled receptors. I. Coupling of alpha 2-adrenergic receptor subtypes to distinct G-proteins. Journal of Biological Chemistry 267(14): 9844-9851, 1992

Factors determining the specificity of signal transduction by guanine nucleotide-binding protein-coupled receptors. III. Coupling of alpha 2-adrenergic receptor subtypes in a cell type-specific manner. Journal of Biological Chemistry 267(33): 24045-24052, 1992

Factors determining the specificity of signal transduction by guanine nucleotide-binding protein-coupled receptors. I: Coupling of α2-adrenergic receptor subtypes to distinct G-proteins. The Journal of Biological Chemistry 267(14): 9844-9851, 1992

Factors determining the specificity of signal transduction by guanine nucleotide-binding protein-coupled receptors. I. Coupling of a2-adrenergic receptor subtypes to distinct G-proteins. The Journal of Biological Chemistry 267: 44-51, 1992

Factors determining the specificity of signal transduction by guanine nucleotide-binding protein-coupled receptors. III. Coupling of a2-adrenergic receptor subtypes in a cell type-specific manner. The Journal of Biological Chemistry 267: 045-52, 1992

Factors determining the specificity of signal transduction by guanine nucleotide-binding protein-coupled receptors. III: Coupling of α2-adrenergic receptor subtypes in a cell type-specific manner. The Journal of Biological Chemistry 267(33): 24045-24052, 1992

Factors determining the specificity of signal transduction by guanine nucleotide-binding protein-coupled receptors. Integration of stimulatory and inhibitory input to the effector adenylyl cyclase. Journal of Biological Chemistry 272(26): 16466-16473, 1997

Identification of a novel guanine nucleotide binding regulatory protein g protein coupled to the alpha adrenergic receptor. Clinical Research 37(2): 267A, 1989

Student-designed, multimedia tutorial on guanine nucleotide-binding protein-coupled receptor signal transduction. FASEB Journal 17(4-5): Abstract 280, 2003

A novel guanine nucleotide-binding protein coupled to the alpha 1-adrenergic receptor. II. Purification, characterization, and reconstitution. Journal of Biological Chemistry 265(31): 18952-18960, 1990

Evidence from photoaffinity labeling studies for coupling of the alpha 2 adrenergic receptor to a guanine nucleotide binding protein. Federation Proceedings 45(6): 1571, 1986

Beta gamma subunits of guanine nucleotide-binding proteins and regulation of spontaneous receptor activity: thermodynamic model for the interaction between receptors and guanine nucleotide-binding protein subunits. Molecular Pharmacology 43(2): 245-256, 1993

A novel guanine nucleotide-binding protein coupled to the alpha 1-adrenergic receptor. I. Identification by photolabeling or membrane and ternary complex preparation. Journal of Biological Chemistry 265(31): 18944-18951, 1990