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Fertility in barley x wheat hybrids hordeum geniculatum all x triticum aestivum their regenerants and hybrid progeny of backcrosses to triticum aestivum l



Fertility in barley x wheat hybrids hordeum geniculatum all x triticum aestivum their regenerants and hybrid progeny of backcrosses to triticum aestivum l



Cereal Research Communications 16(3-4): 157-164



H. geniculatum All .times. T. aestivum L. hybrids were obtained by a methods based on embryo culture. These hybrids showed partial female fertility (0.28% of pollinated florets). Callus tissue was induced from a single young inflorescence on B-5 medium with 3 mg/l 2,4-D. More than 300 plants were regenerated on B-5 medium with 0.5 mg/l kinetin and 0.5 mg/l 2,4-D supplements for a year. These plants were divided into phenotypic classes showing partial female fertility ranging from 0-16.67%. A part of the regenerants was treated with 0.5% colchicine solution containing 4% DMSO. As a result, partially self-fertile plants were produced. An BC1 (H. geniculatum All .times. T. aestivum L) .times. T. aestivum L. plant was studied. Well filled grain was obtained after selfing and pollinating it with wheat pollen.

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