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Fundamental studies on chironomid allergy. III. Allergen analyses of some adult Japanese chironomid midges (Chironomidae, Diptera)



Fundamental studies on chironomid allergy. III. Allergen analyses of some adult Japanese chironomid midges (Chironomidae, Diptera)



Arerugi 37(9): 944-951



Using antisera from experimentally sensitized mice, fractions obtained by gel filtration and anion exchange chromatography of crude extracts, and excretions from 5 species of adult midges of the tribe Chironomini were measured for allergenic activities by PK type skin test on rats. As a result, a high-molecular-weight fraction, obtained by gel filtrations of a crude extract on a Sephadex G-75 followed by a Sephadex G-200 column, accounted for a large portion of the crude extract in protein content and showed a half to twice as high an allergenicity of the crude extract. The molecular weight of the predominant component in this fraction was estimated to be 110K-374K daltons. On the other hand, a component having a molecular weight between 15K and 18K daltons, obtained by fractionations of a crude extract on a Sephadex G-75 and a DE32 column, showed strong allergenicity (3-32 times as allergenic as the crude extract) in any of 5 species. This component was estimated to be a larval Hb, in a monomeric form, which barely persisted without undergoing degradations, on the basis of its marked absorbance at 415 nm. Furthermore, excretions from some species of adult midges showed definite allergenicities, although the strength was only 1/8 to 1/3 of that of the crude extract. This suggests that excretions from adult midges are possible sensitizing agents for humans, originating from chironomids, in our living environments.

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Accession: 007365410

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PMID: 3248028


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