+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Gene encoding the major subunit of CS1 pili of human enterotoxigenic Escherichia coli

Gene encoding the major subunit of CS1 pili of human enterotoxigenic Escherichia coli

Infection and Immunity 58(11): 3594-3600

Some enterotoxigenic strains of Escherichia coli (ETEC) utilize the CS1 pilus for colonization of human intestinal epithelium. We have cloned the gene which encodes the major CS1 subunit and called it cooA (for coli surface antigen one). Hybridization showed that the ETEC strain from which it was cloned carried cooA on a plasmid different from the one encoding its positive regulator, rns. Based on the cooA DNA sequence, cleavage with signal peptidase would be expected to produce a mature protein of 15.2 kDa; a 16-kDa polypeptide that reacted with CS1-specific antiserum was observed on electrophoresis. At the protein level, there was 92% similarity and 55% identity between cooA and cfaB, the major colonization factor antigen I (CFA/I) antigen. However, CS1-specific antisera did not react with CfaB. No hybridization was seen between either of two different cooA probes and total DNA from ETEC strains expressing AFA-1, CFA/I, CS2, CS3, CS4, CS5, or CS6.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 007370817

Download citation: RISBibTeXText

PMID: 1977705

Related references

Sequencing of the gene encoding the major pilin of pilus colonization factor antigen III (CFA/III) of human enterotoxigenic Escherichia coli and evidence that CFA/III is related to type IV pili. Infection and Immunity 63(2): 724-728, 1995

Nucleotide sequence of the gene encoding the major subunit of CS3 fimbriae of enterotoxigenic Escherichia coli. Infection and Immunity 56(12): 3297-3300, 1988

Clone and expression of pili subunit gene from the pathogenic factor adhesion F41 and K99 of the Enterotoxigenic Escherichia coli (ETEC). Chinese Journal of Zoonoses 23(8): 797-800, 2007

Murine immunization with CS21 pili or LngA major subunit of enterotoxigenic Escherichia coli (ETEC) elicits systemic and mucosal immune responses and inhibits ETEC gut colonization. Veterinary Microbiology 202: 90, 2017

The nucleotide sequence of the gene encoding the K88ab protein subunit of procine enterotoxigenic Escherichia coli. Fems Microbiology Letters 12(1): 41-46, 1981

Physical analysis of genes encoding human pyelo nephritis associated pili and common pili of escherichia coli. Abstracts of the Annual Meeting of the American Society for Microbiology 82: ABSTRACT B72, 1982

Structure of the CFA/III major pilin subunit CofA from human enterotoxigenic Escherichia coli determined at 0.90 Å resolution by sulfur-SAD phasing. Acta Crystallographica. Section D Biological Crystallography 68(Pt 10): 1418-1429, 2012

Genes for CS2 pili of enterotoxigenic Escherichia coli and their interchangeability with those for CS1 pili. Infection and Immunity 63(12): 4849-4856, 1995

The structural gene encoding human enterotoxigenic Escherichia coli PCFO20 is homologous to that for porcine 987P. Infection and Immunity 64(4): 1233-1239, 1996

Mutations in the gene encoding the ancillary pilin subunit of the Streptococcus suis srtF cluster result in pili formed by the major subunit only. Plos one 5(1): E8426, 2010

Characterization of new hydrophobic pili of human enterotoxigenic Escherichia coli: a possible new colonization factor. Infection and Immunity 43(3): 959-965, 1984

Sequence analysis of the heat-labile enterotoxin subunit B gene originating in human enterotoxigenic Escherichia coli. Journal of Bacteriology 152(1): 506-509, 1982

The gene encoding the prepilin peptidase involved in biosynthesis of pilus colonization factor antigen III (CFA/III) of human enterotoxigenic Escherichia coli. Microbiology and Immunology 43(9): 853-861, 1999

Purification and characterization of the CS2 pili of colonization factor antigen II produced by human enterotoxigenic Escherichia coli. Microbiology and Immunology 33(4): 265-275, 1989

Construction of Bifidobacterium infantis as a live oral vaccine that expresses antigens of the major fimbrial subunit (CfaB) and the B subunit of heat-labile enterotoxin (LTB) from enterotoxigenic Escherichia coli. Microbiology 158(Pt 2): 498-504, 2012