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Genetic markers for prunus avium l. inheritance and linkage of isozyme loci



Genetic markers for prunus avium l. inheritance and linkage of isozyme loci



Annales des Sciences Forestieres (Paris) 47(2): 131-140



The polymorphism of 10 enzyme systems in wild cherry (Prunus avium L.) was analysed using vertical polyacrylamide gel electrophoresis (AMY, GOT, ME) and isoelectrofocusing (ACP, IDH, LAP, MDH, PGM, SDH, TO) on 286 wild cherries. The products of around 41 loci could be distinguished in these systems, 13 of which displayed polymorphism. The genetics of 7 isozyme loci have been studied using 8 fullsib families: acp1, lap1, sdh1 and mdh1 functioned as monomers, and got1, idh1 and me1 were active as dimers. Two other isozymes (pgm1 and got2) appeared to have simple inheritance, but was not possible to verify this. Joint segregation of 13 locus pairs showed linkage between lap1 and got1 (r = 0.03 .+-. 0.02) and between lap1 and me1 (r = 0.05 .+-. 0.07). ldh1, sdh1, acp1 and mdh1 are not linked to these loci. No linkage has been detected between acp1 and mdh1.

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