Hepatic uptake and biliary secretion of bile acids in the perfused rat liver
Aldini, R.; Roda, A.; Lenzi, P.; Calcaterra, D.; Vaccari, C.; Calzolari, M.; Festi, D.; Mazzella, G.; Bazzoli, F.; Roda, E.
Pharmacological Research 25(1): 51-61
ISSN/ISBN: 1043-6618 PMID: 1738758 DOI: 10.1016/s1043-6618(05)80064-7
Hepatic uptake and biliary secretion have been evaluated in the isolated perfused rat liver for cholic, chenodeoxycholic, ursodeoxycholic acid, both free and taurine-conjugated; the physicochemical properties of the bile acids have also been calculated and related to these experimental parameters. Cholic acid disappearance rate from the perfusate was the fastest, followed by that of ursodeoxycholic and chenodeoxycholic; it was also faster for taurine-conjugated bile acids than for their respective unconjugated forms. The recovery in bile was higher for conjugated than for unconjugated bile acids, and, among each class, was higher for cholic than for chenodeoxycholic and ursodeoxycholic. The hepatic uptake correlated negatively (r = -0.99) with the bile acid lipophilicity, while the biliary secretion correlated with the solubility of the molecules. These results show the effect of the physicochemical properties of BA on their hepatic handling, at the physiological concentration of BA in the portal blood.