+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Human cervical and foreskin epithelial cells immortalized by human papillomavirus dna exhibit dysplastic differentiation in vivo



Human cervical and foreskin epithelial cells immortalized by human papillomavirus dna exhibit dysplastic differentiation in vivo



Cancer Research 50(12): 3709-3715



Human papillomavirus (HPV) DNAs are detected in approximately 90% of anogenital carcinomas. To assess directly the effect of HPV on squamous differentiation, normal human cervical and foreskin epithelial cells and cells immortalized by recombinant HPV DNAs were transplanted beneath a skin-muscle flap in athymic mice. Xenografts containing normal cells formed well-differentiated stratified squamous epithelia 2 to 3 weeks after transplantation, but cell lines immortalized by four HPV types (HPV16, HPV18, HPV31, and HPV33) dectected in anogenital cancer exhibited dysplastic morphology and molecular alterations in gene expression characteristic of intraepithelial neoplasia. Morphological alterations were accompanied by delayed commitment to terminal differentiation, alterations in the pattern of involucrin expression, and reductions in levels of involucrin and keratin 1 RNAs. HPV18-immortalized cells developed dysplastic changes more rapidly than cells immortalized by HPV16 DNA. These results show that human genital epithelial cells immortalized by HPV DNAs detected in genital cancers udnergo dysplastic differentiation in vivo.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 007408779

Download citation: RISBibTeXText

PMID: 1692766


Related references

Hpv immortalized cervical and foreskin epithelial cells exhibit dysplastic differentiation when grafted to nude mice. Howley, P M And T R Broker (Ed ) Ucla (University Of California-Los Angeles) Symposia on Molecular And Cellular Biology New Series, Vol 124 Papillomaviruses; Taos, New Mexico, Usa, March 11-18, 1989 Xxvi+553p Wiley-Liss: New York, New York, Usa; Chichester, England, Uk Illus 231-238, 1990

Human keratinocytes hkc immortalized by human papillomavirus hpv dna associated with cervical cancer exhibit aberrant terminal differentiation. Journal of Cellular Biochemistry Supplement (13 PART C): 216, 1989

Human keratinocytes hkc immortalized by human papillomavirus hpv dnas associated with cervical cancer exhibit aberrant terminal differentiation. Proceedings of the American Association for Cancer Research Annual Meeting 30: 46, 1989

Gene expression profiling of dysplastic differentiation in cervical epithelial cells harboring human papillomavirus 16. Genomics 85(6): 727-738, 2005

Interferon and retinoic acid suppress the growth of human papillomavirus type 16 immortalized cervical epithelial cells, but only interferon suppresses the level of the human papillomavirus transforming oncogenes. Cancer Research 54(8): 2108-2112, 1994

Comparative response of normal and of human papillomavirus-16 immortalized human epithelial cervical cells to benzo[a]pyrene. Oncology Reports 6(6): 1371-1376, 1999

Down-regulation of keratin 14 gene expression after v-Ha-ras transfection of human papillomavirus-immortalized human cervical epithelial cells. Cancer Research 52(21): 5865-5871, 1992

Volume-activated taurine transport is differentially activated in human cervical cancer HT-3 cells but not in human papillomavirus-immortalized Z183A and normal cervical epithelial cells. Clinical & Experimental Pharmacology & Physiology 24(12): 935-939, 1997

Calcium regulates the differentiation of human papillomavirus type 16 (HPV16) immortalized ectocervical epithelial cells, but not the expression of the papillomavirus E6 and E7 oncogenes. Experimental Cell Research 208(1): 161-169, 1993

Human cervical cells immortalized in vitro with oncogenic human papillomavirus DNA differentiate dysplastically in vivo. Gynecologic Oncology 38(3): 407-412, 1990

Retinoid regulation of cell differentiation in a series of human papillomavirus type 16-immortalized human cervical epithelial cell lines. Carcinogenesis 16(2): 375-381, 1995

Retinoic acid inhibits the growth of human papillomavirus immortalized cervical epithelial cells, but only slightly suppresses the level of the papillomavirus transforming genes. Proceedings of the American Association for Cancer Research Annual Meeting 34(0): 544, 1993

Leukoregulin and gamma-interferon inhibit human papillomavirus type 16 gene transcription in human papillomavirus-immortalized human cervical cells. Cancer Research 52(2): 456-463, 1992

Activation of the focal adhesion kinase signal transduction pathway in cervical carcinoma cell lines and human genital epithelial cells immortalized with human papillomavirus type 18. Oncogene. 15(3): 265-274, 1997

Enhanced production of wild-type P53 inhibits growth and differentiation of normal foreskin epithelial cells but not cell lines containing human papillomavirus DNA. Stanley, M A Immunology of human papillomaviruses 9-14, 1994