+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Human muellerian inhibiting substance inhibits tumor growth in vitro and in vivo

Human muellerian inhibiting substance inhibits tumor growth in vitro and in vivo

Cancer Research 51(8): 2101-2106

Mullerian inhibiting substance(MIS) causes regression of the mullerian duct in the male fetus. Bovine MIS has been reported to inhibit the growth of some gynecological tumors. Recombinant human MIS (rhMIS) produced in transfected Chinese hamster ovary cells has been highly purified by immunoaffinity chromatography. The introduction of a salt wash prior to elution of MIS from the affinity column removes a growth-stimulating factor(s) derived from Chinese hamster ovary cells. This immunopurified rhMIS caused significant inhibition (34-59% survival) of A431 (a vulvar epidermoid carcinoma), HT-3 (a cervical carcinoma), HEC-1-A (an endometrial adenocarcinoma), NIH:OVCAR-3 (an ovarian adenocarcinoma), and OM431 (an ocular melanoma) human cell lines in colony inhibition assays. Two cell lines, Hep 3B (a hepatocellular carcinoma) and RT4 (a bladder transitional cell papilloma), were unresponsive to immunopurified rhMIS. Using an in vivo subrenal capsule assay in irradiated CD-1 mice, the growth of A431 and OM431 cells was inhibited by immunopurified rhMIS. We conclude that rhMIS inhibits the growth of certain tumor cell lines in vitro and in vivo.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 007410343

Download citation: RISBibTeXText

PMID: 2009529

Related references

Antibody against a cystic fibrosis transmembrane conductance regulator derived synthetic peptide inhibits anion currents in human colonic cell line t84. Journal of Biological Chemistry 267(12): 8411-8416, 1992

The binding of monomeric and trimeric igg to human macrophages. Federation Proceedings 46(3): 1032, 1987

Insensitivity of the amino acids of canola and rapeseed to methanol ammonia extraction and commercial processing. Food Chemistry 44(4): 283-285, 1992

Recombinant human mouse chimeric monoclonal antibody specific for human adenocarcinoma associated antigen. Hybridoma 10(1): 1-9, 1991

Muellerian inhibiting substance inhibits growth of a human ovarian cancer in nude mice. Annals of Surgery 194(4): 472-480, 1981

Muellerian inhibiting substance inhibits colony growth of a human ovarian carcinoma cell line. Journal of Clinical Endocrinology and Metabolism 54(5): 1051-1055, 1982

Programmed cell death electro physiological and ultrastructural correlations in metamorphosing muscles of lepidopteran insects. Tissue and Cell 11(4): 803-820, 1979

The correlation of follicular fluid cholesterol levels to oocyte fertilization and cleavage in human menopausal gonadotropin human chorionic gonadotropin in vitro fertilization cycles. Journal of In Vitro Fertilization and Embryo Transfer 3(3): 177, 1986

Involution of the chicken thymus and bursa of fabricius during aging. 1979

Recombinant human Clara cell secretory protein in acute lung injury of the rabbit: effect of route of administration. Pediatric Critical Care Medicine 6(6): 698-706, 2005

Muellerian duct regression in the american alligator alligator mississippiensis its morphology and testicular induction. Journal of Experimental Zoology 251(3): 329-338, 1989

The presence of Morus insignis (Moraceae) in the Province of Jujuy (Argentina.). Lilloa 38(2): 173-174, 1995

Human prostatic cells in culture Different testosterone metabolic profile in epithelial cells and fibroblasts from normal or hyperplastic prostates. Journal Of Steroid Biochemistry and Molecular Biology. 58(2): 235-242, 1996

Transfection of the glial cell line-derived neurotrophic factor gene promotes neuronal differentiation. Neural Regeneration Research 9(1): 33-40, 2014

Isolation of the biosynthetic products of the per iodic acid schiff positive pars intermedia cells in the cichlid teleost sarotherodon mossambicus. General and Comparative Endocrinology 49(2): 277-285, 1983