EurekaMag.com logo
+ Site Statistics
References:
53,623,987
Abstracts:
29,492,080
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Identification and classification of potyviruses on the basis of coat protein sequence data and serology



Identification and classification of potyviruses on the basis of coat protein sequence data and serology



Archives of Virology 106(3-4): 171-200



The identification and classification of potyviruses has been in a very unsatisfactory state due to the large size of the group, the apparent vast variation among the members and the lack satisfactory taxonomic parameters that will distinguish distinct viruses from strains. In the past, use of classical methods, such as host range and symptomatology, cross-protection, morphology of cytoplasmic inclusions and conventional serology, revealed a "continuum" implying that the "species" and "strain" concepts cannot be applied to potyvirses. In contrast nucleic acid and amino acid sequence data of coat proteins has clearly demonstrated that potyviruses can be divided into distinct members and strains. This sequence data in combination with information of the structure of the potyvirus particle has been used to develop simple techniques such as HPLC peptide profiling, serology (using polyclonal antibody probes obtained by cross-adsorption with core protein from trypsin treated particles) and cDNA hybridization. These findings, along with immunochemical analyses of overlapping synthetic peptides have established the molecular basis for potyvirus serology; explained many of the problems associated with the application of conventional serology; and provided a sound basis for the identification and classification of potyviruses. As a result, the virus/strain status of some potyviruses has been redefined, requiring a change in the potyvirus nomeclature. These new developments necessiate a re-evaluation of the earlier literature on symptomatology, cross-protection, cytoplasmic inclusion body morphology and serology.

Accession: 007417395

Download citation: RISBibTeXText

PMID: 2673154

DOI: 10.1007/bf01313952

Download PDF Full Text: Identification and classification of potyviruses on the basis of coat protein sequence data and serology



Related references

Coat protein of potyviruses. 5. Symptomatology, serology, and coat protein sequences of three strains of passionfruit woodiness virus. Archives of Virology 102(3-4): 221-232, 1988

Coat protein of potyviruses. 2. Amino acid sequence of the coat protein of potato virus Y. Virology 152(1): 118-125, 1986

Coat protein of potyviruses 3. comparison of amino acid sequence of the coat proteins of four australian strains of sugarcane mosaic virus. Archives of Virology 96(1-2): 59-74, 1987

Further evidence that pepper mottle and potato virus Y are distinct potyviruses Serology and nucleotide sequence of the coat protein gene and 3 untranslated region of pepper mottle virus NC165. Phytopathology 83(12): 1424-1425, 1993

Towards a system for the identification and classification of potyviruses I. Serology and amino acid composition of six distinct viruses. Virology 73(2): 350-362, 1976

Towards a system for the identification and classification of potyviruses part 1 serology and amino acid composition of 6 distinct viruses. Virology 73(2): 350-362, 1976

Amino acid sequence homology of coat proteins as a basis for identification and classification of the potyvirus group. Journal of General Virology 69(11): 2703-2710, 1988

Coat protein of potyviruses. 7. Amino acid sequence of peanut stripe virus. Archives of Virology 119(1-2): 25-35, 1991

Partial sequencing of the genomic RNA of Araujia mosaic virus and comparison of the coat protein sequence with those of other potyviruses. Archives of Virology 152(11): 2125-2129, 2007

Coat protein of potyviruses. 4. Comparison of biological properties, serological relationships, and coat protein amino acid sequences of four strains of potato virus Y. Archives of Virology 102(3-4): 207-219, 1988

A novel approach to the serology of potyviruses involving affinity purified polyclonal antibodies directed towards virus-specific N termini of coat proteins. Journal of General Virology 70(1): 13-23, 1989