EurekaMag.com logo
+ Site Statistics
References:
54,215,046
Abstracts:
30,230,908
PMIDs:
28,215,208
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Identification of protein components of echinococcus granulosus protoscolex antigens for specific serodiagnosis of echinococcus granulosus infection in dogs



Identification of protein components of echinococcus granulosus protoscolex antigens for specific serodiagnosis of echinococcus granulosus infection in dogs



Parasite Immunology (Oxford) 11(3): 279-292



Proteins of Echinococcus granulosus protoscolex excretory/secretory or deoxycholate solubilized somatic antigens were radiolabelled with 125I and immunoprecipitated with sera from dogs naturally or experimentally infected with E. granulosus and various control dog sera. Analysis of immunoprecipitates was performed using one- and two-dimensional gel electrophoresis to identify antigenic protein components specific for E. granulosus. Using both electrophoretic techniques, a basic component of Mr 27 000 and an acidic component of Mr 94 000 were defined in both excretory/secretory and somatic protoscolex antigens, and were specifically identified by 95% and 62% of 21 sera from E. granulosus infected dogs, respectively. An abundant component of Mr 35000 was identified by 100% of these dogs, parts of which were E. granulosus specific. Results of this study should allow identification of specific recombinant antigens for routine serodiagnosis of E. granulosus infection in dogs.

(PDF emailed within 1 workday: $29.90)

Accession: 007421267

Download citation: RISBibTeXText



Related references

Identification of protein components of Echinococcus granulosus protoscolex antigens for specific serodiagnosis of E. granulosus infection in dogs. Parasite Immunology 11(3): 279-291, 1989

Evaluation of a serological test system for the diagnosis of natural echinococcus granulosus infection in dogs using echinococcus granulosus protoscolex and oncosphere antigens. Australian Veterinary Journal 65(12): 369-373, 1988

Evaluation of a serological test system for the diagnosis of natural Echinococcus granulosus infection in dogs using E. granulosus protoscolex and oncosphere antigens. Australian Veterinary Journal 65(12): 369-373, 1988

Use of Echinococcus granulosus worm antigens for immunodiagnosis of Echinococcus granulosus infection in dogs. Veterinary Parasitology 45(1-2): 89-100, 1992

Use of Echinococcus granulosus worm antigens for immunodiagnosis of E. granulosus infection in dogs. Veterinary Parasitology 45(1/2): 89-100, 1992

First molecular evidence of the simultaneous human infection with two species of Echinococcus granulosus sensu lato: Echinococcus granulosus sensu stricto and Echinococcus canadensis. Parasitology Research 115(3): 1065-1069, 2016

The epidemiology of echinococcus granulosus in the uk viii. the structure of adult colonies of echinococcus granulosus equinus say in farm dogs in wales uk. Annals of Tropical Medicine and Parasitology 85(1): 63-74, 1991

Recognition of several antigens by monoclonal antibodies against protoscolex of Echinococcus granulosus. Chinese Journal of Veterinary Science and Technology 23(10): 23, 1993

Differential serodiagnosis for cystic and alveolar echinococcosis using fractions of Echinococcus granulosus cyst fluid and E multilocularis protoscolex. American Journal of Tropical Medicine & Hygiene 60(2): 188-192, 1999

A recombinant antigen with potential for serodiagnosis of Echinococcus granulosus infection in dogs. International Journal for Parasitology, 207: 943-950, 1990

Echinococcus granulosus 14-3-3 protein: a potential vaccine candidate against challenge with Echinococcus granulosus in mice. Biomedical and Environmental Sciences 25(3): 352-358, 2012

Studies on excretory-secretory (ES) antigens of Echinococcus granulosus: the immunogenicity of ES antigens of the protoscolex. Chinese Journal of Veterinary Science and Technology 21(11): 8-10, 1991

The distribution and prevalence of Echinococcus granulosus (Batsch, 1786) and other parasites of dogs in Kayseri region. II. Prevalence, health and economic importance of Echinococcus granulosus. Turkiye Parazitoloji Dergisi 17(3/4): 83-87, 1993

Differential serodiagnosis for cystic and alveolar echinococcosis using fractions of Echinococcus granulosus cyst fluid (antigen B) and E. multilocularis protoscolex (EM18). American Journal of Tropical Medicine and Hygiene 60(2): 188-192, 1999

The epidemiology of Echinococcus infection in Great Britain. II. The incidence of Echinococcus granulosus and some Other cestodes hi farm dogs in mid-Wales. Ann. trop. Med. Parasit, 58: 147-152, 1964