+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Immunization of mice with recombinant vaccinia virus expressing authentic dengue virus nonstructural protein NS1 protects against lethal dengue virus encephalitis



Immunization of mice with recombinant vaccinia virus expressing authentic dengue virus nonstructural protein NS1 protects against lethal dengue virus encephalitis



Journal of Virology 64(9): 4356-4363



The protective immunity conferred by a set of recombinant vaccinia viruses containing the entire coding sequence of dengue virus type 4 nonstructural glycoprotein NS1 plus various flanking sequences was evaluated by using a mouse encephalitis mode. Mice immunized with recombinant vNS1-NS2a, which expresses authentic NS1, were solidly protected agianst intracerebral dengue virus challenge. However, mice immunized with recombinants vNS1-15%NS2a and vRSVG/NS1-15%NS2a, which express aberrant forms of NS1, were only partially protected (63 to 67% survival rate). Serologic analysis showed that mice immunized with vNS1-NS2a developed high titers of antibodies to NS1 as measured by radioimmunoprecipitation, enzyme-linked immunosorbent assay, and complement-mediated cytolytic assays. In addition, a pool of sera from these animals was protective in a passive transfer experiment. Lower titers of NS1-specific antibodies were detected in sera of animals immunized with vNS1-15%NS2a or vRSVG/NS1-15% NS2a by all three assays. These data support the view that protection against dengue virus infection in mice may be mediated at least in part by NS1-specific antibodies through a mechanism of complement-mediated lysis of infected cells. Additionally, immunization with two recombinant viruses expressing authentic NS1 of dengue virus type 2 conferred partial protection (30-50%) against dengue virus type 2 challenge.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 007425856

Download citation: RISBibTeXText

PMID: 2143542


Related references

Mice immunized with recombinant vaccinia virus expressing dengue 4 virus structural proteins with or without nonstructural protein NS1 are protected against fatal dengue virus encephalitis. Journal of Virology 63(6): 2853-2856, 1989

Immunization of mice with dengue structural proteins and nonstructural protein NS1 expressed by baculovirus recombinant induces resistance to dengue virus encephalitis. Journal of Virology 62(8): 3027-3031, 1988

Immunization with recombinant vaccinia viruses expressing structural and part of the nonstructural region of tick-borne encephalitis virus cDNA protect mice against lethal encephalitis. Journal of Biotechnology 44(1-3): 97-103, 1996

Immunization of rhesus monkeys with a recombinant of modified vaccinia virus Ankara expressing a truncated envelope glycoprotein of dengue type 2 virus induced resistance to dengue type 2 virus challenge. Vaccine 18(27): 3113-3122, 2000

A MVA construct expressing a secretable form of the Dengue virus 3 envelope protein protects immunized mice from dengue-induced encephalitis. Vaccine 34(50): 6120-6122, 2016

Modified vaccinia virus Ankara immunization protects against lethal challenge with recombinant vaccinia virus expressing murine interleukin-4. Journal of Virology 78(22): 12471, 2004

Chimeric tick-borne encephalitis /dengue virus or recombinant TBE-vaccinia virus protects against TBE in a murine model. American Journal of Tropical Medicine and Hygiene 47(4 Suppl. ): 102-103, 1992

Expression of dengue virus structural proteins and nonstructural protein NS1 by a recombinant vaccinia virus. Journal of Virology 61(12): 4019-4022, 1987

The chimeric Japanese encephalitis/Dengue 2 virus protects mice from challenge by both dengue virus and JEV virulent virus. Protein and Cell 8(3): 225-229, 2017

DNA immunization with recombinant dengue 2 virus nonstructural protein NS1 elicits protective immunity in mice. Zhonghua Weishengwuxue He Mianyixue Zazhi 22(1): 33-36, 2002

Immunization with a vaccinia recombinant expressing the F protein protects rabbits from challenge with a lethal dose of rinderpest virus. Virology 170(1): 11-18, 1989

Dengue 2 virus envelope protein expressed by a recombinant vaccinia virus fails to protect monkeys against dengue. Journal of General Virology 69: 1921-1929, 1988

Protection of mice against dengue 2 virus encephalitis by immunization with the dengue 2 virus non-structural glycoprotein NS1. Journal of General Virology 68: 853-857, 1987

Immunization with a recombinant fowlpox virus expressing a hepatitis C virus core-E1 polyprotein variant, protects mice and African green monkeys (Chlorocebus aethiops sabaeus) against challenge with a surrogate vaccinia virus. Biotechnology and Applied Biochemistry 51(Pt 2): 97-105, 2008

Protection against lethal Japanese encephalitis virus infection of mice by immunization with the highly attenuated MVA strain of vaccinia virus expressing JEV prM and E genes. Vaccine 17(3): 261-268, 1999