Improved high performance liquid chromatographic method for the quantification of 3 methylhistidine in serum and urine

Fermo I.; De Vecchi E.; Arcelloni C.; Brambilla P.; Pastoris A.; Paroni R.

Journal of Liquid Chromatography 14(9): 1715-1728


ISSN/ISBN: 0148-3919
DOI: 10.1080/01483919108049649
Accession: 007435095

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We optimized an high-performance liquid chromatographic (HPLC) method for the determination of 3-methylhistidine (3-MeHis) in biological fluids. After pre-column derivatization with OPA, with OPA, analytical separation was achieved on a reversed-phase C18 column by a simple gradient between sodium propionate buffer and acetonitrile. The method is accurate, reproducible and sensitive, and allows the determination of urinary 3-MeHis levels in about 55 min. Other additional 16 amino acids may be easily quantified while the 3-Me-His peak is well resolved from an unknown urinary compound potentially interfering.