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In vitro capacitation and fertilizing ability of rabbit spermatozoa treated with lysophosphatidylcholine



In vitro capacitation and fertilizing ability of rabbit spermatozoa treated with lysophosphatidylcholine



Japanese Journal of Fertility & Sterility 35(1): 209-212



We examined effects of lysophosphatidylcholine (LPC) on the incidence of the acrosome reaction and fertilizing ability of ejaculated rabbit spermatozoa. When rabbit spermatozoa were preincubated in a modified Tyrode's solution for 3 hours and then were exposed to various concentration solutions of LPC (10, 25, 50, or 100 .mu.g/ml) for 15 minutes, the acrosome reaction of spermatozoa was facilitated markedly. However, at 100 .mu.g/ml of LPC the sperm motility was injured considerably. The fertilizing ability of spermatozoa treated with LPC also was tested, in vitro fertilization of rabbit eggs ovulated. From results obtained it was confirmed that ejaculated rabbit spermatozoa treated with LPC had high fully capacity to fertilize eggs.

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