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In vitro studies on the early stage of rabbit blastocyst implantation



In vitro studies on the early stage of rabbit blastocyst implantation



Japanese Journal of Fertility & Sterility 34(2): 120-127



To elucidate the mechanism of implantation, an in vitro experimental model was carried out using rabbits. Blastocytes obtained 6 1/2 days after mating were placed on cultured endometrial cells and cultured for 3, 6, and 12 hours. The blastocysts and the endometrial cells were studied by light and electron microscopy and the following results were obtained. After 3 hours in culture, cytoplasmic projections from the endometrial cells had thrust through the zona pellucida, and particles of toluidine blue stain were observed in the blastocysts. After 6 hours, the protuberances of the endometrial cell layer, which contained large amounts of collagen fibrils, were observed around the blastocyst attachment, and large numbers of lysosomes and glycogen granules were present in the endometrial cells. After 12 hours, the zona pellucida had become very thin and coarse. The most characteristic finding was the existence of a layer of substance between the endometrial cells and the zona pellucida (SEZ).

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Accession: 007440550

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