In vivo characterisation of extracellular dopamine, GABA and acetylcholine from the dorsolateral striatum of awake freely moving rats by chronic microdialysis
Osborne, P.G.; O'Connor, W.T.; Kehr, J.; Ungerstedt, U.
Journal of Neuroscience Methods 37(2): 93-102
ISSN/ISBN: 0165-0270 PMID: 1679148 DOI: 10.1016/0165-0270(91)90119-k
Basal extracellular (EC) DA, 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), gamma aminobutyric acid (GABA) and acetylcholine (ACh) were measured in dialysates from the dorsolateral striatum (DLS) of awake rats, every 30 min for 4.5 h each day over a 4-day period. The responsiveness of basal EC DA, DOPAC, HVA and GABA to local perfusion with tetrodotoxin (1 .mu.m) was measured 1 and 4 days after implantation. In addition, EC ACh was also measured 4 days after probe implantation. The results of this study indicate that EC levels of DA, DOPAC, HVA, GABA and ACh can be reliably monitored for up to 4 days after probe implantation. In addition, we show that striatal EC levels of DA, GABA and ACh may be regarded as a reflection of ongoing neuronal activity for up to 4 days after implantation of a microdialysis probe.