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Induction of growth factor-receptor and metalloproteinase genes by epidermal growth factor and/or transforming growth factor-alpha in human gastric carcinoma cell line MKN-28



Induction of growth factor-receptor and metalloproteinase genes by epidermal growth factor and/or transforming growth factor-alpha in human gastric carcinoma cell line MKN-28



Japanese Journal of Cancer Research 81(8): 793-798



We examined the effects of epidermal growth factor (EGF) and transforming growth factor-.alpha. (TGF-.alpha.) on EGF receptor (EGFR) phosphorylation and the expression of mRNAs for oncogenes, growth factors, their receptors and metalloproteinase genes by MKN-28 gastric carcinoma cells which express EGF, TFG-.alpha. and EGFR genes. Both EGF and TGF-.alpha. stimulated EGFR phosphorylation. EGF and TGF-.alpha. induced FOS, MYC and ERBB-2 oncogene expression. Interestingly, EGF increased the expression of mRNAs for TGF-.alpha. and EGFR. On the other hand, TGF-.alpha. increased TGF-.alpha. mRNA but decreased the expression of mRNAs for EGFR and TGF-.beta. Furthermore, mRNAs for interstitial collagenase, stromelysin and procollagen type I genes were also enhanced for treatment with EGF and TGF-.alpha. These results indicate that EGF and TGF-.alpha. successively evoke cascade phenomena which favor tumor progression, invasion and extracellular matrix formation, acting as autocrine growth regulators for gastric carcinomas.

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Accession: 007450682

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PMID: 2168868


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