Section 8
Chapter 7,455

Influence of branched chain amino acids on the change of aromatic amino acid levels under the perfusion of rat liver

Moriai, O.

Journal of the Iwate Medical Association 42(1): 67-78


Accession: 007454440

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The influence of branched chain amino acid (BCAA: valine, leucine, isoleucine) on the changes in aromatic amino acid (AAA: phenylalanine, tyrosine, tryptophan) concentrations in the perfusate under the perfusion of rat liver was investigated. The concentrations of AAA in the perfusate which was perfused by Krebs-Ringer solution with or without BCAA and/or AAA for 90 minutes were measured with a Hitachi amino acid autoanalyzer, and the histological findings of lysosomes in the hepatocyte after perfusion were also observed by a Hitachi electron microscopy. Although the uptake of AAA into the liver was not influenced by the addition of BCAA, the release of AAA into the perfusate was significantly suppressed (P < 0.01). Compared with the perfusion by Krebs Ringer solution with BCAA and that by Krebs Ringer solution alone, there were clearly fewer autolysosomes in the hepatocyte after perfusion by Krebs-Ringer solution with BCAA. These findings suggest that the addition of BCAA may inhibit the catabolism of protein in the rat liver, regulating the release of AAA from that organ.

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