Influence of branched chain amino acids on the change of aromatic amino acid levels under the perfusion of rat liver
Journal of the Iwate Medical Association 42(1): 67-78
The influence of branched chain amino acid (BCAA: valine, leucine, isoleucine) on the changes in aromatic amino acid (AAA: phenylalanine, tyrosine, tryptophan) concentrations in the perfusate under the perfusion of rat liver was investigated. The concentrations of AAA in the perfusate which was perfused by Krebs-Ringer solution with or without BCAA and/or AAA for 90 minutes were measured with a Hitachi amino acid autoanalyzer, and the histological findings of lysosomes in the hepatocyte after perfusion were also observed by a Hitachi electron microscopy. Although the uptake of AAA into the liver was not influenced by the addition of BCAA, the release of AAA into the perfusate was significantly suppressed (P < 0.01). Compared with the perfusion by Krebs Ringer solution with BCAA and that by Krebs Ringer solution alone, there were clearly fewer autolysosomes in the hepatocyte after perfusion by Krebs-Ringer solution with BCAA. These findings suggest that the addition of BCAA may inhibit the catabolism of protein in the rat liver, regulating the release of AAA from that organ.