+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Interleukin 1 beta increases the cytosolic free sodium concentration in isolated rat islets of Langerhans



Interleukin 1 beta increases the cytosolic free sodium concentration in isolated rat islets of Langerhans



Scandinavian Journal of Immunology 32(1): 53-58



Interleukin 1 (IL-1) exerts both stimulatory and inhibitory (cytotoxic) effects on insulin-producing .beta. cells in isolated pancreatic islets. Since alteration in ion fluxes is crucial for endocrine cell activation and is a denominator of cell death, and since IL-1 was recently shown to increase the total sodium content in a murine pre-B-lymphocyte cell line, we investigated the effect of recombinant human IL-1.beta. (rhIL-1.beta.) on the cytosolic free sodium concentration (fNai+) in rat islets. Furthermore, long-term rhIL-1.beta. effects on islet cell function were studied during exposure of islets to amiloride, a blocker of the plasma membrane Na+/H+ exchange. One hour of islet exposure to 60 U/ml of rhIL-1.beta. caused a threefold increase in fNai+ in islet cells, and this effect was abolished by depletion of extracellular sodium. Blockade of Na+/H+ exchange with amiloride abolished the inhibitory effect of rhIL-1.beta. on insulin release. In conclusion, rhIL-1.beta. was found to increase sodium influx in pancreatic islet cells. This might underlie the widespread effects of rhIL-1.beta. on .beta.-cell function and morphology, possibly related to IL-1-mediated toxic free radical formation.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 007476445

Download citation: RISBibTeXText

PMID: 2200107


Related references

Interleukin 1 beta increases the cytosolic sodium concentration in isolated pancreatic islets. Diabetologia 33(Suppl.): A19, 1990

Interleukin 1 effect on isolated rat islets of langerhans impact of beta cell activity and sodium proton exchange. Scandinavian Journal of Immunology 30(5): 638, 1989

Stimulation by thrombin increases the cytosolic free Na+ concentration in human platelets. Studies with the novel fluorescent cytosolic Na+ indicator sodium-binding benzofuran isophthalate. Journal of Biological Chemistry 265(32): 19543-19550, 1990

Interaction of beta-cell activity and IL-1 concentration and exposure time in isolated rat islets of Langerhans. Diabetes 38(10): 1211-1216, 1989

Modulation of calcium flux influences interleukin 1 beta effects on insulin release from isolated islets of Langerhans. Acta Endocrinologica 121(3): 447-455, 1989

Identification of alpha- and beta-cells in intact isolated islets of Langerhans by their characteristic cytoplasmic Ca2+ concentration dynamics and immunocytochemical staining. Diabetes 47(5): 751-757, 1998

The role of nitric oxide on the functional change of interleukin-1 beta and interleukin-6 induced rat pancreatic islets of Langerhans. Zhonghua Nei Ke Za Zhi 38(7): 458-461, 1999

Characterization of acetylcholine-induced increases in cytosolic free calcium concentration in individual rat pancreatic beta-cells. Cell Adhesion and Communication 1(4): 343-353, 1994

Interleukin-1 beta-induced formation of EPR-detectable iron-nitrosyl complexes in islets of Langerhans. Role of nitric oxide in interleukin-1 beta-induced inhibition of insulin secretion. Journal of Biological Chemistry 266(32): 21351, 1991

Adenosine increases intracellular Ca2+ concentration in mouse islets of Langerhans via ATP production. Diabetes 48(Suppl. 1): A247, 1999

Changes in cytosolic free calcium concentration in isolated rat parotid cells by cholinergic and beta-adrenergic agonists. Biochemical and Biophysical Research Communications 131(3): 1048-1055, 1985

Erythropoietin increases cytosolic free calcium concentration and thrombin induced changes in cytosolic free calcium in platelets from spontaneously hypertensive rats. Biochemical and Biophysical Research Communications 177(3): 991-997, 1991

Interleukin 1 induces new protein formation in isolated rat islets of Langerhans. European Journal of Endocrinology 121(1): 136-140, 1989

Interleukin 1 induced new protein formation in isolated rat islets of langerhans. Acta Endocrinologica 121(1): 136-140, 1989

Heat shock increases cytosolic free calcium concentration via sodium calcium exchange in human epidermoid a 431 cells. American Journal of Physiology 263(1 Part 1): C30-C38, 1992