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Intermediary metabolism and thymol formation in in vitro cultures of carum copticum

Intermediary metabolism and thymol formation in in vitro cultures of carum copticum

Biotechnology and Applied Biochemistry 14(3): 256-264

Four specific stages of in vitro cultures, from callus to plantlet, were obtained from Carum copticum with varying morphogenesis and different levels of differentiation and thymol content, 2,4-Dichlorophenoxyacetic acid gave rise to only unorganized callus proliferation, while indole-3-acetic acid and .alpha.-naphthaleneacetic acid promoted chlorophyll formation as well as semiorganization and benzylaminopurine triggered plantelet formation. Correlation between secondary metabolite formation and primary metabolism was examined in those cultures with 14C-labelled Kreb's cycle intermediates, e.g., acetate, glucose, aspartate, and malate. The incorporation of 14C into the organized cultures was low in amino acid-, organic acid-, and ethanol-insoluble fractions (protein and carbohydrate), and high in lipid, total volatile, and thymol fractions. This trend was reversed in unorganized white callus, indicating greater synthesis of volatile compounds in semiorganized cultures than in unorganized cultures. The thymol content increased five times upon chlorophyll formation/semiorganization. The biosynthetic ability of in vitro cultures to produce thymol and plantelet regeneration is established in C. copticum.

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