+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Intertypic genomic rearrangements of poliovirus strains in vaccinees



Intertypic genomic rearrangements of poliovirus strains in vaccinees



Virology 167(2): 507-514



In vivo intertypic RNA recombination has previously been observed in excreted tyep 3 poliovirus isolates from a normal asymptomatic primary vaccinee. This study examines isolates from additional primary vaccinees to determine whether intertypic recombination is a general occurrence in excreted polioviruses. T1 RNase oligonucleotide fingerprinting and limited dideoxy primer extension RNA sequencing demonstrated no evidence of intertypic recombination among type 1 or type 2 excreted strains. However, vaccinees secreting type 3 strains for long periods of time eventually produced recombinant strains involving either type 1 or type 2 poliovirus. Moreover, a characteristic time course of appearance of excreted type 3 intertypic recombinant polioviruses was established. Type 3/type 1 and type 3/type 2 recombinant strains appeared at Days 10-11 with a single crossover site in the gene for nonstructural protein 2C. Type 3/type 2/type 3 complex recombinant strains with an additional crossover site in the polymerase gene replaced type 3/type 2 strains ar approximately Day 28. A significant portion of the genome of the type 32 Sabin vaccine strain is thus replced during long-term excretion by vaccinees, and the appearance of some genomic arrangements coincided with a base deletion at the 3' terminus.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 007479322

Download citation: RISBibTeXText

PMID: 2849237

DOI: 10.1016/0042-6822(88)90113-4


Related references

Genomic features of intertypic recombinant sabin poliovirus strains excreted by primary vaccinees. Journal of Virology 75(13): 5740-5751, 2001

Evaluation of Sabin live poliovirus vaccine in Japan. IV. Marker tests on poliovirus strains recovered from vaccinees and their contacts. Japanese Journal of Medical Science and Biology 20(2): 167-173, 1967

Serodifferentiation of type 3 poliovirus strains isolated 1960-1965 from patients and healthy vaccinees. Characterization of strains associated with disease in vaccinated persons and household contacts. American Journal of Epidemiology 87(2): 396-410, 1968

Intertypic recombinations between vaccine poliovirus strains isolated from poliomyelitis patients. Molekulyarnaya Genetika Mikrobiologiya i Virusologiya (11): 14-20, 1989

Evaluation of Sabin live poliovirus vaccine in Japan: V Neurovirulence of virus strains derived from vaccinees and their contacts. Jap J Med Sci Biol 26(4): 281-302, 1967

Neurovirulence of the intertypic poliovirus recombinant v3/a1-25: characterization of strains isolated from the spinal cord of diseased monkeys and evaluation of the contribution of the 3' half of the genome. Journal of General Virology 66: 309-316, 1985

Studies on the recombination between RNA genomes of poliovirus: the primary structure and nonrandom distribution of crossover regions in the genomes of intertypic poliovirus recombinants. Virology 161(1): 54-61, 1987

Genomic modifications in Sabin vaccine strains isolated from vaccination-associated cases, healthy contacts and healthy vaccinees. Acta Virologica 40(3): 157-170, 1996

Poliovirus In Febrile Vaccinees. Archives of Environmental Health 10: 16-20, 1965

Construction of poliovirus intertypic recombinants by use of cDNA. Journal of Virology 57(3): 1187-1190, 1986

Intertypic recombination in poliovirus genetic and biochemical studies. Virology 124(1): 121-132, 1983

Enhanced detection of poliovirus in stool of vaccinees by RT/PCR. Abstracts of the Interscience Conference on Antimicrobial Agents & Chemotherapy 35: 185, 1995

Intertypic antigenic determinants of intracellular proteins of different poliovirus serotypes. Voprosy Virusologii .(5): 570-573, 1980

Intertypic cross-reactions of nonneutralizing, monoclonal poliovirus antibodies. Journal of Virology 49(3): 1002-1004, 1984

Genomic rearrangements in Neisseria meningitidis strains of the ET-5 complex. Current Microbiology 40(6): 372-379, 2000