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Intracellular transport of recombinant coronavirus spike proteins: implications for virus assembly

Intracellular transport of recombinant coronavirus spike proteins: implications for virus assembly

Journal of Virology 64(1): 339-346

Coronavirus spike protein genes were expressed in vitro by using the recombinant vaccinia virus expression system. Recombinant spike proteins were expressed at the cell surface and induced cell fusion in a host-cell-dependent fashion. The intracellular transport of recombinant spike proteins was studied. The half time of acquisition of resistance to endo-.beta.-N-acetylglucosaminidase H was approximately 3h for the recombinant feline infectious peritonitis virus S protein. The S protein in feline infectious peritonitis virus-infected cells was found to have a half time of acquisition of resistance to endo-.beta.-N-acetylglucosaminidase H of approximately 1h. This difference can be explained by the fact that coronavirus budding take place at intracellular membranes and that the oligosaccharides of the spike protein are modified after budding. Apparently, spike protein incorporated into budded virions is transported faster through the Golgi apparatus than is spike protein alone. These findings provide new insights into the mechanism of coronavirus budding and are discussed in relation to current models of intracellular transport and sorting of proteins.

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Accession: 007481068

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PMID: 2403441

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