Isoflavone glycoside formation in transformed and non transformed suspension and hairy root cultures of lupinus polyphyllus and lupinus hartwegii
Berlin, J.; Fecker, L.; Ruegenhagen, C.; Sator, C.; Strack, D.; Witte, L.; Wray, V.
Zeitschrift fuer Naturforschung Section C Journal of Biosciences 46(9-10): 725-734
Transformed cell suspension and hairy root cultures were established by infecting seedlings of Lupinus polyphyllus and L. hartwegii with various wild type strains of Agrobacterium tumefaciens and A. rhizogenes. Transformation of the cultures was confirmed either by their photohormone autotrophy, detection of opines or southern anlysis. Glucosides of genistein and 2'-hydroxygenistein, were found to be the main secondary metabolites in normal and transformed suspension cultures as well in hairy root cultures, Although some of the isoflavone glycosides of the cultures were apparently new constituents of Lupinus, they were afterwards also found in young seedlings.