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Isolation and characterization of human peripheral blood cd4 positive t cell clones expressing gamma delta t cell receptors



Isolation and characterization of human peripheral blood cd4 positive t cell clones expressing gamma delta t cell receptors



Immunology Letters 29(3): 197-204



Rare T cell clones bearing both CD4 and .gamma.delta. T cell receptors (TcR.gamma.delta.) were obtained from human peripheral blood by cell sorting using anti-CD4 and anti-TCR.delta.1 antibodies. All the clones established were reactive with anti-TcR.gamma.delta.1 antibody, whereas only about 20% of the clones showed reactivity with anti-.delta. TCS1 antibody. Unlike most CD4+ T cells bearing TcR.alpha.beta., all the clones tested showed lectin-dependent and anti-CD3 antibody-redirected cytolytic activity. About 60% of the clones exhibited natural killer cell-like activity. Immunoprecipitation analysis of TcR.gamma.delta. showed that each clone expressed either a disulfide-linked or non-disulfide-linked heterodimer consisting of 37-44-kDa TcR.gamma. and TcR.delta. chains.

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