+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Isolation propagation and characterization of some strains of rotavirus from neonatal bovine calves in india



Isolation propagation and characterization of some strains of rotavirus from neonatal bovine calves in india



Indian Journal of Animal Sciences 61(5): 463-467



Rotavirus was isolated in cell-culture from the faeces of cattle and buffalo calves positive for rotavirus by polyacrylamide-gel electrophoresis. The isolation was made in MA 104 cell-line in the presence of trypsin. The cytopathic effect was of cells and ultimately detached from glass surface. The isolates were confirmed as rotavirus by electron microscopy, dot-ELISA, polyacrylamide-gel electrophoresis of genome RNA and serum-neutralization tests. All of them were typical rotaviruses as revealed by RNA electrophorotyping.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 007496146

Download citation: RISBibTeXText


Related references

Molecular characterization of group A rotavirus (RVA) strains detected in bovine and porcine species: Circulation of unusual rotavirus strains. A study from western, India. Acta Virologica 63(1): 103-110, 2019

Evidence of bovine non-group A rotavirus in diarrhoeic neonatal calves in India. Veterinary Record 149(12): 364-365, 2001

Isolation of bovine rotavirus in cell culture from neonatal calves with diarrhoea. 2007

Evidence for Interstate Transmission and Increase in Prevalence of Bovine Group B Rotavirus Strains with a Novel Vp7 Genotype among Diarrhoeic Calves in Eastern and Northern States of India. Epidemiology and Infection 135(8): 1324-1330, 2007

Evidence for interstate transmission and increase in prevalence of bovine group B rotavirus strains with a novel VP7 genotype among diarrhoeic calves in Eastern and Northern states of India. Epidemiology and Infection 135(8): 1324-1330, 2007

Isolation, characterization, and serial propagation of a bovine group C rotavirus in a monkey kidney cell line (MA104). Journal of Clinical Microbiology 29(11): 2609-2613, 1991

Generation of monoclonal antibodies neutralizing bovine rotavirus for therapeutic assays in neonatal calves experimentally infected with rotavirus. Annales de Medecine Veterinaire 131(4): 275-286, 1987

Characterization of nontypeable rotavirus strains from the United States: identification of a new rotavirus reassortant (P2A(6),G12) and rare P3(9) strains related to bovine rotaviruses. Virology 294(2): 6-69, 2002

Characterization of nontypeable rotavirus strains from the United States: identification of a new rotavirus reassortant (P2A[6],G12) and rare P3[9] strains related to bovine rotaviruses. Virology 294(2): 256-269, 2002

Modification of rotavirus multiplex RT-PCR for the detection of G12 strains based on characterization of emerging G12 rotavirus strains from South India. Journal of Medical Virology 79(9): 1413-1421, 2007

Full Genome Characterization of Novel DS-1-Like G8P[8] Rotavirus Strains that Have Emerged in Thailand: Reassortment of Bovine and Human Rotavirus Gene Segments in Emerging DS-1-Like Intergenogroup Reassortant Strains. Plos one 11(11): E0165826, 2016

Molecular characterization of unusual bovine group A rotavirus G8P[14] strains identified in western India: emergence of P[14] genotype. Veterinary Microbiology 148(2-4): 384-388, 2011

Molecular characterization of rare bovine group A rotavirus G15P[11] and G15P[21] strains from eastern India: identification of simian SA11-like VP6 genes in G15P[21] strains. Virus Genes 37(2): 241-249, 2008

Isolation of cytopathogenic Rotavirus from neonatal calves. Acta Microbiologica Academiae Scientiarum Hungaricae 26(3): 225-231, 1979

Molecular characterization of a rare G1P[19] rotavirus strain from India: evidence of reassortment between human and porcine rotavirus strains. Journal of Medical Microbiology 58(Pt 12): 1611-1615, 2009