EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Lactate transport in skeletal muscle cells: uptake in L6 myoblasts






Acta Physiologica Scandinavica 141(3): 379-381

Lactate transport in skeletal muscle cells: uptake in L6 myoblasts

During exercise, lactate is produced by degradation of glucose-6-phosphate during glycolysis in the contracting muscles. This lactate is metabolized during and after exercise in the muscle itself and also in the liver and other muscles, which can use it as an energy metabolite or can resynthetize glycogen. Lactate is transported in the blood, and the rate of muscular utilization may be limited by two factors: the rate of metabolis utilization by the muscle cell; and the rate of transport across the membrane regulating lactate transfer from the blood to the cell. We have studied lactate uptake in L6 muscle cells by incorporation of 14C-lactate. The uptake rates were linear for 20 seconds with 5 mM lactate and 10 seconds with 20 mM. The uptake during 10 seconds for physiological lactate concentrations (1-20 mM) gave a straight line passing through the origin. Lactate uptake was not altered by specific inhibitors or lactate transport (2.5 mM .alpha. cyano-4-hydroxycinnamic acid. 5 .mu.M 4,4'-diisothiocyanostilbene-2,2'-disulphonic acid) or by the stereospecific D-lactate inhibitor. The results suggest that L-lactate uptake in L6 cells occurs by passive diffusion.

Accession: 007504180

PMID: 1858508

DOI: 10.1111/j.1748-1716.1991.tb09094.x

Download PDF Full Text: Lactate transport in skeletal muscle cells: uptake in L6 myoblasts



Related references

Asymetry of lactate transport in skeletal muscle cells. FASEB Journal 13(4 PART 1): A396, March 12, 1999

Skeletal muscle Pi transport and cellular [Pi] studied in L6 myoblasts and rabbit muscle-membrane vesicles. Biochimica et Biophysica Acta 1137(1): 10-18, 1992

Comparison of RRR-alpha- and all-rac-alpha-tocopherol uptake by permanent rat skeletal muscle myoblasts (L6 cells): effects of exogenous lipoprotein lipase. Lipids 33(10): 1001-1008, 1998

Lactate uptake by skeletal muscle effects of metabolic rate and lactate concentration. Medicine & Science in Sports & Exercise 24(5 SUPPL): S108, 1992

Blood lactate concentration and lactate uptake by contracting canine skeletal muscle. Medicine & Science in Sports & Exercise 23(4 SUPPL): S36, 1991

Lactate transport during differentiation of skeletal muscle cells: evidence for a specific carrier in L6 myotubes. Acta Physiologica Scandinavica 144(4): 469-471, 1992

Effect of lactate concentration and metabolic rate on net lactate uptake by canine skeletal muscle. American Journal of Physiology 266(4 Pt 2): R1095-R1101, 1994

Blood flow and a v lactate difference during lactate uptake by in situ canine skeletal muscle. Medicine & Science in Sports & Exercise 21(2 SUPPL): S35, 1989

Current aspects of lactate exchange: Lactate/H+ transport in human skeletal muscle. European Journal of Applied Physiology 86(1): 12-16, November, 2001

Current aspects of lactate exchange: lactate/H+ transport in human skeletal muscle. European Journal of Applied Physiology 86(1): 12-16, 2002