EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Lactate utilization by an in situ perfused trout heart effects of workload and blockers of lactate transport


, : Lactate utilization by an in situ perfused trout heart effects of workload and blockers of lactate transport. Journal of Experimental Biology 155: 357-374

Lactate utilization was studied in an in situ perfused trout heart preparation that was capable of performing at levels similar to in vivo maximum cardiac performance. Hearts were perfused with modified Cortland saline containing 0.5 mmol l-1 iodoacetic acid (to block endogenous glycolysis) and varying amounts of lactate (1 or 10 mmol l-1). We confirmed previous observations that lactate utilization is limited by substrate availability. However, contrary to previous observations, exogenous fuel availability did not limit cardiac performance, even at the high workload. Furthermore, when plentiful (i.e. 10 mmol l-1), exogenous lactate was preferred over endogenous fuel and was able to supply the heart's energy requirements at both the low and high workloads. Pyruvate at 10-fold greater concentration, had no apparent effect on lactate utilization at the high workload. .alpha.-Cyano-4-hydroxycinnamate (.alpha.-CIN) (2.5 mmol l-1) proved to be an unsuitable probe of lactate transport in the trout heart as it caused a reduction in both lactate utilization and cardiac performance. However, addition of 20 .mu.mol l-1 isobutyl carbonyl lactyl anhydride or 100 .mu.mol l-1 4-acetamido-4'-isothiocyanostilbene-2,2'-disulphonic acid (putative lactate transport blockers) to the perfusate virtually abolished lactate oxidation at the high workload without affecting cardiac performance. These observations suggest that lactate uptake by the in situ perfused trout heart is carrier-mediated.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 007504182

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Sunder, R; Ayengar, Knn; Rangaswami, S., 1976: Structures of four new triterpenes from the rhizomes of Polypodium juglandifolium. Journal of the Chemical Society, Perkin Transactions 1 1: 117

King C.M., 1983: The relationships between beech nothofagus sp seedfall and populations of mice mus musculus and the demographic and dietary responses of stoats mustela erminea in 3 new zealand forests. There was a significant relationship between seedfall and populations of mice in all forests studied. Good seadfalls were followed by increases in density of mice and changes in population structure not seen in years when the seedfall failed. Pred...

Anonymous, 2008: Northwest Forest Plan - the first 10 years 1994-2003 Socioeconomic monitoring of the Okanogan-Wenatchee National Forest and five local communities. This case study was developed to respond to two socioeconomic effectiveness monitoring questions posed in the Northwest Forest Plan Record of Decision (ROD). The first focuses on use levels of natural resources: Are predictable levels of timber a...

Elstner, Ef, 1973: The asymmetry of the tricarboxylic acid cycle, demonstrated by studies on the biosynthesis of the antibiotic showdomycin. Berichte der Deutschen Botanischen Gesellschaft: 6 (5 9) 381-392

Guo TongBin; Wang ZhenYing; Liang Bo; Yang ZhongLin; Qian GuiZhi; Zhou Yong; Yu AiXiang; Huang XiuHua, 2000: Bionomics of Micromelalopha troglodyta. Micromelalopha troglodyta [Notodontidae] is the main foliage pest of Populus spp. in Xuzhou district, Jiangsu Province, China, where it has 5 generations a year and overwinters as pupae. The first generation is a population accumulating stage, the...

Dehouck, Benedicte; Dehouck, Marie Pierre; Fruchart, Jean Charles; Cecchelli, Romeo, 1993: A new function for the LDL receptor Transcytosis of LDL through the blood-brain barrier. Circulation 88(4 PART 2): I465

Stearn, Benjamin F.; Polis, Michael A., 1994: Prophylaxis of opportunistic infections in persons with HIV infection. BACKGROUND: Morbidity and mortality associated with human immunodeficiency virus infection and the acquired immunodeficiency syndrome is most often related to associated opportunistic infections. OBJECTIVE: To review the prophylaxis of common oppo...

Steudel, K., 1980: New estimates of early hominid body size. Body weights for 12 early hominid specimens are estimated based on an analysis of 4 variables [palate breadth, bizygomatic breadth, orbital width and femoral circumference] shown to have high correlation with body size in living Old World primates...

Madden, A. D.; Haggis, M. J.; Holt, J., 1993: Helicoverpa armigera oviposition on cotton in the Sudan Gezira associated with rainfall. Helicoverpa armigera egg counts made in 1973 and 1975 from cotton fields within four blocks of the Sudan Gezira were compared with rainfall information from a network of rain gauges. Aspects of rainfall in the 6 days before an egg count were analy...

Young W.L.; Massaro A.; Ornstein E.; Ostapkovich N.; Prohovnik I.; Mohr J.P., 1990: Comparison of carbon dioxide reactivity for middle cerebral artery velocities and xenon 133 cerebral blood flow values during anesthesia. Anesthesiology (Hagerstown) 73(3A): A205