+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Lysosomes in the pathogenesis of liver injury in chronic copper poisoned sheep: an ultrastructural and morphometric study



Lysosomes in the pathogenesis of liver injury in chronic copper poisoned sheep: an ultrastructural and morphometric study



Journal of Comparative Pathology 100(4): 381-390



Chronic copper poisoning was induced in sheep by oral dosing with CuSO4. The distribution of copper between hepatocytes was unequal and, with increasing liver copper concentration, isolated hepatocytes packed with electron-dense lysosomes were seen. These cells underwent degeneration and necrosis. During the pre-haemolytic period, the concentration of Cu in the liver increased and the volume density, numerical density and mean volume of hepatocyte lysosomes increased in a linear fashion, indicating that there was proliferation as well as increase in the size of lysosomes. However, in animals killed during haemolysis, the numerical density had decreased but the volume density was little changed which indicates that lysosomal production may have diminished. It is postulated that the necrosis of hepatocytes packed with electron-dense lysosomes may be due to the accumulation of toxic amounts of copper in the cytosol, resulting from a reduced uptake of copper into the lysosomal system of these cells, and that the susceptibility of liver cells to Cu-induced damage may be increased if lysosome production is diminished.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 007527831

Download citation: RISBibTeXText

PMID: 2760272

DOI: 10.1016/0021-9975(89)90003-0


Related references

An ultrastructural and morphometric study of the liver of normal and copper-poisoned sheep. American Journal of Pathology 99(2): 429-450, 1980

Effects of 2 mercaptoethanol on the solubility of copper and zinc containing proteins in liver samples from normal and chronic copper poisoned sheep. Acta Veterinaria Scandinavica 25(1): 10-20, 1984

An ultrastructural study of the kidney of normal, copper poisoned and thiomolybdate-treated sheep. Journal of Comparative Pathology 96(6): 593-612, 1986

Histochemical detection of copper in the liver from sheep chronically poisoned by copper. Acta Veterinaria Scandinavica 10(3): 288-291, 1969

Concentration of selenium in liver in relation to copper level in normal and copper-poisoned sheep. Acta Veterinaria Scandinavica 19(3): 472-474, 1978

The concentrations of molybdenum and zinc in liver in relation to copper accumulation in normal and copper poisoned sheep. Acta Veterinaria Scandinavica 17(3): 307-315, 1976

Solubility of copper and zinc containing proteins before and after 2-mercaptoethanol treatment of liver samples from normal and from chronically copper poisoned sheep. Acta Pharmacologica et Toxicologica 55(3): 247-251, 1984

Morphometric study of ultrastructural changes induced in rat liver by chronic alcohol intake. American Journal of Digestive Diseases 18(5): 398-402, 1973

Structural changes in the kidney of chronic copper poisoned sheep. Braetter, P And P Schramel (Ed ) Trace Element Analytical Chemistry in Medicine And Biology, Vol 2 Proceedings Of The International Workshop, Neuherberg, West Germany, April 1982 Xvi+1189p Walter De Gruyter: Berlin, West Germany; New York, N Y , Usa Illus P341-352, 1983

Simultaneous effects of dietary molybdenum and copper on the accumulation of copper in the liver and kidney of copper poisoned rats a histochemical study. Industrial Health 16(3-4): 119-126, 1978

Chronic copper toxicity of sheep: an ultrastructural study of spongiform leucoencephalopathy. Research in Veterinary Science 15(1): 88-95, 1973

Studies on the pathogenesis of hepatolenticular degeneration. 3. The effect of copper on rat liver lysosomes. American Journal of Pathology 53(6): 903-927, 1968

An investigation of the effects of intravenous administration of thiomolybdate on copper metabolism in chronic Cu-poisoned sheep. British Journal of Nutrition 46(3): 469-480, 1981

Chronic copper poisoning in sheep. III. The distribution of soluble copper- and zinc-binding liver proteins of lambs compared with adult sheep. Acta Pharmacologica et Toxicologica 40(5): 570-574, 1977

Distribution of soluble copper binding and zinc binding proteins in liver and kidney of chronic copper poisoned goats. Acta Veterinaria Scandinavica 21(4): 578-586, 1980