+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Membrane integration and intracellular transport of the coronavirus glycoprotein E1, a class III membrane glycoprotein



Membrane integration and intracellular transport of the coronavirus glycoprotein E1, a class III membrane glycoprotein



Journal of Biological Chemistry 263(29): 14956-14963



The E1-glycoprotein (Mr = 26,014; 228 amino acids) of mouse hepatitis virus A59 is a class III membrane glycoprotein which has been used in this study as a model system in the study of membrane integration and protein transport. The protein lacks an NH2-terminal cleavable signal sequence and spans the viral membrane three times. Hydrophobic domains I and III could serve as signal sequences for cotranslational membrane integration. Domain I alone was sufficient to translocate the hydrophilic NH2 terminus of E1 across the membranes as evidenced by glycosylation of a newly introduced N-glycosylation site. The COOH-terminal part of E1 involving amino acids Leu124 to Thr228 was found to associate tightly with membranes at the post-translational level, although this part of the molecule lacks pronounced hydrophobic sequences. Membrane protection assays with proteinase K showed that a 2-kDa hydrophilic fragment was removed from the COOH terminus of E1 indicating that the protein is largely embedded into the membrane. Microinjection of in vitro transcribed capped and polyadenylated mRNA into CV-1 cells or into secretory AtT20 pituitary tumor cells showed that the E1-protein accumulated in the Golgi but was not detectable at the plasma membrane or in secretory granules. The 28 NH2-terminal hydrophilic amino acid residues play no role in membrane assembly or in intracellular targeting. Various NH2-terminal portions of E1 were fused to Ile145 of the cytoplasmic N-protein of mouse hepatitis virus. The resulting hybrid proteins were shown to assemble into membranes in vitro and were detected either in the rough endoplasmic reticulum or transient vesicles of microinjected cells.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 007544111

Download citation: RISBibTeXText

PMID: 2844793


Related references

Expression of MHV-A59 M glycoprotein: effects of deletions on membrane integration and intracellular transport. Advances in Experimental Medicine and Biology 276: 127-135, 1990

Sequence and topology of a model intracellular membrane protein, E1 glycoprotein, from a coronavirus. Nature 308(5961): 751-752, 1984

Intracellular retention of the e1 glycoprotein from an avian coronavirus involves the first membrane spanning domain. Journal of Cellular Biochemistry Suppl. (14 Part C): 46, 1990

Signal recognition particle-dependent insertion of coronavirus E1, an intracellular membrane glycoprotein. Journal of Biological Chemistry 260(8): 4648-4652, 1985

Membrane glycoprotein sorting role of the cytoplasmic tail and transmembrane domain of hsv glycoprotein gb 1 in intracellular targetting. Journal of Cellular Biochemistry Suppl. (11 Part A): 263, 1987

Biosynthesis of a membrane glycoprotein and its intracellular transport to the prelysosomal compartment. Journal of Cell Biology 115(3 Part 2): 229A, 1991

Biosynthesis and intracellular transport of a membrane glycoprotein (plgp57) of the prelysosome compartment. Molecular Membrane Biology 11(2): 127-134, 1994

Identification of the membrane-active regions of the severe acute respiratory syndrome coronavirus spike membrane glycoprotein using a 16/18-mer peptide scan: implications for the viral fusion mechanism. Journal of Virology 79(3): 1743-1752, 2005

Membrane glycoprotein folding, oligomerization and intracellular transport: effects of dithiothreitol in living cells. Embo Journal 12(5): 2151-2157, 1993

Acquired thrombopathy associated with autoantibodies against membrane glycoprotein iib iiia complex 1 membrane glycoprotein analysis. Blut 60(2): 125, 1990

Post-translational processing of an O-glycosylated protein, the human CD8 glycoprotein, during the intracellular transport to the plasma membrane. Journal of Biological Chemistry 267(35): 25196-25201, 1992

Biosynthesis and intracellular transport of the melanosomal membrane glycoprotein gp75 the human b brown locus gene product. Journal of Cell Biology 115(3 Part 2): 474A, 1991

Intracellular transport of the major glycoprotein of zymogen granule membranes in the rat pancreas. Demonstration of high turnover at the plasma membrane. European Journal of Biochemistry 144(1): 177-183, 1984

Structure and biogenesis of coronavirus membrane glycoprotein e 1. Journal Of Cellular Biochemistry Supplementrt D: 276, 1986

Effects of deletions in the carboxy-terminal hydrophobic region of herpes simplex virus glycoprotein gB on intracellular transport and membrane anchoring. Journal of Virology 67(8): 4856-4866, 1993