Mobilization of agrobacterium rhizogenes root inducing plasmids into rhizobium meliloti rhizobium galegae and rhizobium leguminosarum biovar trifolii

Novikova, N.I.; Pavlova, E.A.; Safronova, V.I.; Zabelina, N.K.

Genetika 27(2): 228-237


ISSN/ISBN: 0016-6758
Accession: 007556889

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Plasmids of Agrobacterium rhizogenes strains 8196 and 15834 were marked with Tn5-mob transposon and transferred into non-pathogenic soil bacterium A. radiobacter. Recombinants of A. radiobacter with pRi: :Tn5-mob induced "hairy root" syndromes on tobacco leaves and carrot disks. These strains were used as donors in crosses with Rhizobium strains specific for Medicago, Trifolium and Galega. Symbiotic properties of Rhizobium transconjugants were tested with legume plants. All strains were virulent. R. galegae (pRi) transconjugants appeared to form uneffective symbiosis with legume plants and have root-inducing activity on tobacco leaves. trifolii (pRi) transconjugants induced hairy roots on tobacco leaves and formed nodules on clover plants with reduced nitrogen fixation. There was evidence that the level of acetylene reduction was decreased three weeks after inoculation. R. meliloti (pRi) transconjugants were characterized by an increased virulence on alfalfa, as compared with parental strain, while they induced no hairy roots on tobacco leaves in an aseptic culture. These recombinants were stable and retained pRi after reisolation from nodules. The role of plasmids in evolution of Rhizobium is discussed.