Molecular cloning of a lupinus polyphyllus complementary dna encoding a basic peroxidase isoenzyme of cell suspension cultures
Perrey, R.; Warskulat, U.; Wink, M.
Journal of Plant Physiology 137(5): 537-540
1991
ISSN/ISBN: 0176-1617 DOI: 10.1016/s0176-1617(11)80695-4
Accession: 007563285
We isolated and sequenced a cDNA clone from a cell suspension culture of Lupinus polyphryllus, which encodes about 54% (701 bp) of a basic perioxidase isoenzyme. The nucleotide sequence is 51.8% homologous to a peroxidase gene of tobacco and the predicted amino acid sequence shows up to 80% homology to peroxidase-specific domains of the already known isoenzymes fron turnip, horseradish and tobacco. Expression studies indicate that the mature lupin peroxidase mRNA is approximately 1.3 kb in size and is expressed in suspension-cultured cells and in leaf and root tissue of intact lupin plants, but not in the stem tissue.