+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Molecular cloning, sequencing and expression in Escherichia coli of the bean yellow mosaic virus coat protein gene



Molecular cloning, sequencing and expression in Escherichia coli of the bean yellow mosaic virus coat protein gene



Journal of General Virology 70: 1961-1974



The sequence of 1015 nucleotides from the 3' poly(A) tract of the potyvirus bean yellow mosaic virus (BYMV) RNA has been determined from two cDNA clones. This sequence contained a single long open reading frame (ORF) starting upstream of the cloned region. The ORF was expressed as a fusion protein in Escherichia coli, and the product was detected by antibodies specific for the coat protein of BYMV. The predicted length of the coat protein gene was 822 nucleotides, corresponding to a 273 amino acid coat protein of Mr 30910. The deduced amino acid sequence of the BYMV coat protein was compared to the chemically determined amino acid composition of purified virion protein, and of protein prepared from trypsin-treated virions. The nucleotide and deduced amino acid sequences were compared to the sequences of the coat protein genes of other potyviruses. The BYMV coat protein gene was found to be 50 to 61% homologous to those of other potyviruses at both the nucleotide and amino acid levels: the greatest variation was between the 5'-proximal one-fifth of the genes. Amino acid sequences and hydrophilicity plots of the different potyvirus coat proteins showed similarities which indicated that the structure of the coat protein is highly conserved; a non-terminal region of variability was predicted to be exposed on the exterior of the virion. A putative cleavage site at a glutamine-serine dipeptide was identified by similarity in context to the cleavage sites of tobacco etch virus and tobacco vein mottling virus coat proteins from the viral polyproteins. The BYMV 3'-terminal non-coding region of 166 nucleotides is followed by a poly(A) tract.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 007563768

Download citation: RISBibTeXText

PMID: 2671258

DOI: 10.1099/0022-1317-70-8-1961


Related references

Molecular cloning, sequencing and expression in Escherichia coli of the odontoglossum ringspot virus coat protein gene. Journal of General Virology 72: 2247-2249, 1991

Complementary dna cloning and sequencing of the bean yellow mosaic virus coat protein gene and comparison with other potyviral capsid proteins. Phytopathology 78(12 PART 1): 1518, 1988

Cloning and expression of coat protein gene of watermelon mosaic virus 2 in Escherichia coli. Journal of Fruit Science 13(1): 5-10, 1996

Cloning of coat protein gene of soybean mosaic virus and its expression in Escherichia coli. Chinese Journal of Biotechnology 9(3): 143-149, 1993

Cloning and expression of the coat protein gene of Barley yellow dwarf virus-PAV in Escherichia coli. Iranian Journal of Biotechnology 2(2): 84-89, 2004

Molecular cloning and nucleotide sequencing of the 3'-terminal region of a South African isolate of ryegrass mosaic virus RNA and in vitro expression of the coat protein gene. Archives of Virology 141(1): 185-195, 1996

Cloning and sequencing of coat protein gene of Zucchini yellow mosaic virus isolated from squash and muskmelon in Turkey. Turkish Journal of Biology 36(4): 423-429, 2012

Molecular cloning, sequencing and expression of CP gene of Barley stripe mosaic virus China strain (BSMV-CH) in Escherichia coli BL21 and its antiserum preparation. Acta Phytopathologica Sinica 36(5): 397-403, 2006

Molecular cloning of the coat protein gene of a singapore isolate of zucchini yellow mosaic virus. Phytopathology 82(6): 722, 1992

Properties of viruses of the potyvirus group. 2. Buoyant density, S value particle morphology, and molecular weight of the coat protein subunit of bean yellow mosaic virus, pea mosaic virus, lettuce mosaic virus, and potato virus YN. Netherlands Journal of Plant Pathology 80(1): 19-27, 1974

Molecular cloning and nucleotide sequence of the coat protein gene of a Cuban isolate of potato leafroll virus and its expression in Escherichia coli. Virus Genes 9(1): 77-83, 1994

Molecular cloning and sequencing of outer capside protein gene of rice dwarf virus and its expression in Escherichia coli. Weishengwu Xuebao 39(4): 305-314, 1999

Molecular cloning and sequencing of outer capsid protein gene of rice dwarf virus and its expression in Escherichia coli. Wei Sheng Wu Xue Bao 39(4): 305-314, 2003

Bean yellow mosaic virus coat protein and gusA gene expression in transgenic Gladiolus plants. Acta Horticulturae ( 447): 393-399, 1997

Molecular cloning and nucleotide sequence of the coat protein gene of the NL-3 and type strains of bean common mosaic virus. Phytopathology 82(10): 1111, 1992