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Monoclonal antibody Br4 recognizes specific neuronal cell types



Monoclonal antibody Br4 recognizes specific neuronal cell types



Journal of Neuroscience Research 29(1): 107-113



A monoclonal antibody termed as Br4 was prepared by a fusion of the myeloma P3 .times. 63-AG8-653 with spleen cells from BALB/c mice immunized with chick embryonic brain cells. Immunocytochemically, it reacts strongly with certain neurons in cerebral cortex, hippocampus, substantia nigra, and granular layer of cerebellum from brain but does not react with the white matter. A monoclonal antibody Br4 also reacts with primary cultured chick neurons, but not with cultured astrocytes. Western blots show that Br4 recognized three proteins of 145,000 108,000 and 97,000 molecular weight from the rat brain. The protein with molecular weight 145,000 (p145) and the protein with 97,000 molecular weight (p145) and the protein with 97,000 molecular weight (p97) are essentially soluble; p145 is especially enriched in the synaptosomal soluble fraction. The protein with 180,000 molecular weight (108) is found in both membrane-bound and soluble forms. Western blots show that among the tissues examined the three proteins are found most abundant in brain and especially enriched in the cerebral cortex and hippocampus. The Br4 antigens may be useful in identifying neuronal cell subpopulations in the central nervous system.

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Accession: 007566804

Download citation: RISBibTeXText

PMID: 1715925

DOI: 10.1002/jnr.490290112


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