EurekaMag
+ Translate
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Morganella morganii urease: purification, characterization, and isolation of gene sequences


Morganella morganii urease: purification, characterization, and isolation of gene sequences



Journal of Bacteriology 172(6): 3073-3080



ISSN/ISBN: 0021-9193

PMID: 2345135

DOI: 10.1128/jb.172.6.3073-3080.1990

Morganella morganii, a very common cause of catheter-associated bacteriuria, was previously classified with the genus Proteus on the basis of urease production. M. morganii constitutively synthesizes a urease distinct from that of other uropathogens. The enzyme, purified 175-fold by passage through DEAE-Sepharose, phenyl-Sepharose, Mono-Q, and Superose 6 chromatography resins, was found to have a native molecular size of 590 kilodaltons and was composed of three distinct subunits with apparent molecular sizes of 63, 15, and 6 kilodaltons, respectively. Amino-terminal analysis of the subunit polypeptides revealed a high degree of conservation of amino acid sequence between jack bean and Proteus mirabilis ureases. Km for urea equalled 0.8 mM. Antiserum prepared against purified enzyme inhibited activity by 43% at a 1:2 dilution after 1 h of incubation. All urease activity was immunoprecipitated from cytosol by a 1:16 dilution. Antiserum did not precipitate ureases of other species except for one Providencia rettgeri strain but did recognize the large subunits of ureases of Providencia and Proteus species on Western blots (immunoblots). Thirteen urease-positive cosmid clones of Morganella chromosomal DNA shared a 3.5-kilobase (kb) BamHI fragment. Urease gene sequences were localized to a 7.1-kb EcoRI-SalI fragment. Tn5 mutagenesis revealed that between 3.3 and 6.6 kb of DNA were necessary for enzyme activity. A Morganella urease DNA probe did not hybridize with gene sequences of other species tested. Morganella urease antiserum recognized identical subunit polypeptides on Western blots of cytosol from the wild-type strain and Escherichia coli bearing the recombinant clone which corresponded to those seen in denatured urease. Although the wild-type strain and recombinant clone produced equal amounts of urease protein, the clone produced less than 1% of the enzyme activity of the wild-type strain.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 007568056

Download citation: RISBibTeXText

Related references

Morganella morganii urease purification and cloning of gene sequences. Abstracts of the Annual Meeting of the American Society for Microbiology 89: 41, 1989

Isolation, purification, and further characterization of an L-phenylalanine oxidase from Morganella morganii. Applied Biochemistry and Biotechnology 48(2): 61-74, 1994

Isolation, purification, and further characterization of an L-phenylalanine oxidase from Morganella morganii. Applied Biochemistry and Biotechnology 48(2): 61-74, 1994

Purification and characterization of chloramphenicol acetyltransferase from Morganella morganii. Journal of Biochemistry and Molecular Biology 34(5): 415-420, 2001

Purification and characterization of esterase from Morganella morganii ZJB-09203. Sheng Wu Gong Cheng Xue Bao 30(1): 129-138, 2014

Purification and Characterization of a Novel Carbonyl Reductase from Morganella morganii J-8. Chinese Journal of Biotechnology 23(2): 268-272, 2007

Purification and characterization of carbonyl enantioselective reductase from Morganella morganii J-8. Sheng Wu Gong Cheng Xue Bao 23(2): 268-272, 2007

Purification and Biochemical Characterization of a Tyrosine Phenol-lyase from Morganella morganii. Applied Biochemistry and Biotechnology 192(1): 71-84, 2020

Recognition of Morganella subspecies, with proposal of Morganella morganii subsp. morganii subsp. nov. and Morganella morganii subsp. sibonii subsp. nov. International Journal of Systematic Bacteriology 42(4): 613-620, 1992

Sequencing, characterization, and gene expression analysis of the histidine decarboxylase gene cluster of Morganella morganii. Current Microbiology 68(3): 404-411, 2014

A bifunctional urease enhances survival of pathogenic Yersinia enterocolitica and Morganella morganii at low pH. Journal of Bacteriology 178(22): 6487-6495, 1996

Characterization of In3Mor, a new integron carrying VIM-1 metallo-beta-lactamase and sat1 gene, from Morganella morganii. Journal of Antimicrobial ChemoTherapy 59(4): 739-741, 2007

Production and purification of L-phenylalanine oxidase from Morganella morganii. Bioseparation 2(3): 147-154, 1991

Isolation of Morganella morganii from a domestic rabbit with bronchopneumonia. Veterinary Record 161(15): 530-531, 2007

Purified ureases from helicobacter pylori morganella morganii and jack bean and sequenced urease genes of proteus mirabilis suggest a common genetic origin. Abstracts of the Annual Meeting of the American Society for Microbiology 90: 64, 1990