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Mutations necessary for the residence of rp4 in xanthomonas campestris pathovar citri



Mutations necessary for the residence of rp4 in xanthomonas campestris pathovar citri



Current Microbiology 19(4): 217-222



The broad-host-range plasmid RP4 was conjugatively transferred from Escherichia coli to Xanthomonas campestris pv. citri strain XAS4501. Low transfer frequency of RP4 (about 6.2 .times. 10-7) into Xanthomonas and deletion of RP4 plasmid in some of the transconjugants have been observed. The deletion regions have been mapped. RP4 and the deletion mutants were genetically studied in both E. coli and Xanthomonas. Since RP4.DELTA.11, one of the deletion types, was transferred by pRK2013 about 200-fold higher than the self-transmission of RP4 into Xanthomonas, a theoretical transmission frequency in range 10-3 was postulated for RP4. By using cured RP4-harboring Xanthomonas strains as recipient, the expected transfer frequencies have been obtained. The results revealed (i) mutation(s) in RP4 (deletion or point mutation) or in host may be necessary for the residence of RP4 replicons in Xanthomonas, and (ii) the wild-type RP4 harboring Xanthomonas are, but not restrictionless, mutants. A mutation in transcription or translation associated with the regulation of RP4 genes in Xanthomonas has been suggested.

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