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Novel and sensitive noncompetitive enzyme immunoassay for kassinin in rat plasma



Novel and sensitive noncompetitive enzyme immunoassay for kassinin in rat plasma



Experientia 45(5): 470-472



A novel and sensitive noncompetitive enzyme immunoassay for kassinin is described. Kassinin was biotinylated using sulfosuccinimidyl-6-(biotinamido)hexanoate. The biotinylated kassinin was trapped on anti-kassinin IgG-coated polystyrene balls, and, after washing to eliminate other biotinylated substances, was eluted with HCl. The biotinylated kassinin eluted was reacted with anti-kassinin Fab'-peroxidase conjugate and trapped onto streptavidin-coated polystyrene balls. Peroxidase activity bound to the polystyrene balls was assayed by fluorimetry. The detection limit of kassinin was 0.13 pg (0.1 fmol)/tube or 0.065 .mu.g/l of rat plasma, which was 750-fold or 15-fold lower than that for competitive radioimmunoassay.

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Accession: 007602580

Download citation: RISBibTeXText

PMID: 2656287

DOI: 10.1007/bf01952033


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