Optimal conditions for the production of aspergillus fumigatus aspergillus niger and aspergillus flavus antigens for the serological diagnosis of aspergillosis

Perez Mejia, A.; Toriello, C.

Revista Mexicana de Micologia 5: 261-272


Accession: 007619354

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Metabolic (culture filtrate), polysaccharide and cytoplasmic antigens of Aspergillus fumigatus, A. niger and A. flavus were obtained at 2, 4, 8 and 15 days of fungal growth. No antigens were isolated after 3 weeks to eliminate non-specific reactions due to C carbohydrate-C reactive protein. Their immunological reactivity was evaluated and compared by gel immunodiffusion and counterimmunoelectrophoresis with anti-A. fumigatus, anti-A. niger and anti-A. flavus hyperimmune rabbit antisera. Fifteen days cytoplasmic antigens from the three fungal species, showed a good reactivity and no cross reactions when tested with sera from patients with other mycotic diseases.