EurekaMag
+ Translate
+ Most Popular
Sobre os generos Lopadusa Stal e Bothrocoris Mayr (Hemiptera, Pentatomidae, Pentatomini)
RFEF pilot plant for inactivation of Escherichia coli in apple juice
Calpionella alpina Lorenz als "Leitfossil"
Sooretama: The Atlantic Rain Forest of Brazil. Francis Dov Por
Novel approaches in myeloma therapy
Preliminary Notes on the Study of Mitochondria During the 1st Stages of the Destruction of Cadavers
A preliminary study of the biology of lichee stink bug, Tessaratoma papulosa Drury, and its control
The status of the Sabu Island python - Die Situation des Savu-Pythons
Randomised trial of intravenous immunoglobulin as prophylaxis against infection in plateau-phase multiple myeloma. the UK Group for Immunoglobulin Replacement therapy in Multiple Myeloma
Conflicts between children
Clinical presentations in neuropsychiatry
Method of suturing a perforated ulcer of the stomach and duodenum
Identification of mechanical asphyxiation in cases of attempted masking of the homicide
Effects of endogenous ascorbate on oxidation, oxygenation, and toxicokinetics of cell-free modified hemoglobin after exchange transfusion in rat and guinea pig
Is it warm? Is it real? Or just low spatial frequency?
Additions and corrections to the list of Korean Heteroptera
Factors contributing to the maintenance of oligotrophy in an alpine glacial moraine lake (Wallowa lake) in Northeast Oregon
Classification, paleoecology, and biostratigraphy of crinoids from the Stull Shale (Late Pennsylvanian) of Nebraska, Kansas, and Iowa
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Neue Formen der Bauchsammler aus Turkmenien (Hymenoptera, Apoidea)
Atypische Hühnerpest und Newcastle-Krankheit. Ein Identifizierungsversuch
Parasitellus fucorum in the debris of Apis mellifera - a risk of confusion with Tropilaelaps spp
Applying the Technology Acceptance Model to the introduction of healthcare information systems
Ein akarologischer Nachruf für den "Roland"
Mutation research on crop plants in Argentina

Partial characterization of pseudomonas fluorescens ssp cellulosa endoglucanase activity produced in escherichia coli


Partial characterization of pseudomonas fluorescens ssp cellulosa endoglucanase activity produced in escherichia coli



Journal of Industrial Microbiology 5(2-3): 59-64



ISSN/ISBN: 0169-4146

The recombinant plasmid, pPFC4, which carries Pseudomonas fluorescens subsp. cellulosa chromosomal DNA was previously isolated on the basis of its ability to direct the expression of endoglucanase in Escherichia coli. In the present study, some physical and chemical properties of this activity were characterized. The major portion (78.4%) of the endoglucanase activity is found in the periplasmic space of E. coli. This plasmid-encoded endoglucanase has a pH optimum of approximately 6.0 and a temperature optimum of approximately 50.degree. C. With carboxymethylcellulose-zymograms, after polyacrylamide gel electrophoresis, periplasmic extracts from E. coli carrying pPFC4 show six distinct bands with endoglucanase activity. The molecular mass of the major endoglucanase band is approximately 29 kDa while the remaining bands with endoglucanase activity range from 48 to 100 kDa. Although the basis of this heterogeneity is not known, the DNA insert of pPFC4 that encodes endoglucanase activity is not large enough to contain six separate genes; hence, the observed array of endoglucanases may result from post-translantion modification of one or two primary gene products.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 007632388

Download citation: RISBibTeXText

Related references

Characterization of an Endoglucanase from Pseudomonas fluorescens subsp. cellulosa Produced in Escherichia coli and Regulation of the Expression of Its Cloned Gene. Applied and Environmental Microbiology 54(2): 302-308, 1988

Characterization and expression in Escherichia coli of an endoglucanase gene of Pseudomonas fluorescens subsp. cellulosa. Biochimica et Biophysica Acta 950(2): 204-214, 1988

Characterization and expression in Escherichia coli of an endoglucanase gene of Pseudomonas fluorescens subsp. cellulosa. Biochimica et Biophysica Acta (Bba) - Gene Structure and Expression 950(2): 204-214, 1988

Cloning of an endoglucanase gene from pseudomonas fluorescens var cellulosa into escherichia coli and pseudomonas fluorescens. Journal of Industrial Microbiology 1(2): 79-86, 1986

Purification of pseudomonas fluorescens ssp cellulosa endoglucanases produced in escherichia coli. Current Microbiology 20(5): 339-342, 1990

DNA sequence analysis of endoglucanase genes from Pseudomonas fluorescens subsp. cellulosa and Pseudomonas sp. NCIB 8634. Journal of Industrial Microbiology 6(4): 285-290, 1990

Isolation of Endoglucanase Genes from Pseudomonas fluorescens subsp. cellulosa and a Pseudomonas sp. Applied and Environmental Microbiology 51(6): 1367-1369, 1986

Expression of an endoglucanase gene of pseudomonas fluorescens var cellulosa in zymomonas mobilis. FEMS Microbiology Letters 49(3): 363-366, 1988

Partial characterization of an inducible amylase produced by Pseudomonas cellulosa. Applied Biochemistry and Biotechnology 50(3): 243-250, 1995

The amino terminal region of an endoglucanase from pseudomonas fluorescens ssp cellulosa constitutes a cellulose binding domain that is distinct from the catalytic center. Molecular Microbiology 4(5): 759-768, 1990

The N-terminal region of an endoglucanase from Pseudomonas fluorescens subspecies cellulosa constitutes a cellulose-binding domain that is distinct from the catalytic centre. Molecular Microbiology 4(5): 759-767, 1990

Cloning of an endoglucanase gene from Pseudomonas fluorescensvar.cellulosainto Escherichia coliand Pseudomonas fluorescens. Journal of Industrial Microbiology and Biotechnology 1(2): 79-86, 1986

Initial characterization of a bolA homologue from Pseudomonas fluorescens indicates different roles for BolA-like proteins in P. fluorescens and Escherichia coli. Fems Microbiology Letters 262(1): 48-56, 2006

Characterization of the gene celD and its encoded product 1,4-beta-D-glucan glucohydrolase D from Pseudomonas fluorescens subsp. cellulosa. Biochemical Journal 285: 947-955, 1992

Characterization of the gene celD and its encoded product 1,4-β-D-glucan glucohydrolase D from Pseudomonas fluorescens subsp. cellulosa. Biochemical Journal (London 1984) 285: 947-955, 1992