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Phosphorus 32 and biotin labelled in vitro transcribed complementary rna probes for the detection of potato spindle tuber viroid and chrysanthemum stunt viroid



Phosphorus 32 and biotin labelled in vitro transcribed complementary rna probes for the detection of potato spindle tuber viroid and chrysanthemum stunt viroid



Research in Virology 141(1): 97-108



Replacing nick-translated DNA probes by in vitro transcribed complementary RNA (cRNA) probes considerably increased the sensitivity of dot-blot detection tests of potato spindle tuber viroid and chrysanthemum stunt viroid. As compared to the limit of detection of 5-10 pg of viroid obtained with 32P-labelled DNA probes, cRNA probes allow the detection of less than 1 pg of pure viroid. When labelled with biotin by incorporation of biotin-labelled ribonucleotides, the cRNA probes have a limit of detection of approximately 5 pg of purified viroid.

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