Plant regeneration from callus and protoplast cultures of lotus pedunculatus cav

Pupilli, F.; Arcioni, S.; Damiani, F.; Pezzotti, M.

Plant Cell Tissue and Organ Culture 23(3): 193-200

1990


ISSN/ISBN: 0167-6857
DOI: 10.1007/bf00034431
Accession: 007660032

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Abstract
Plant regeneration from leaf- and cotyledon-derived calli and from protoplast-derived tissue has been obtained in Lotus pedunculatus. Callus induction was achieved with 2,4-D and plant regeneration required the following two media sequences: bud formation was stimulated by IAA and BA and shoot growth by kinetin. Root formation occurred in the presence of IAA. Cotyledon protoplasts showed a low plating efficiency and plant regeneration was achieved via an intervening callus phase.

Plant regeneration from callus and protoplast cultures of lotus pedunculatus cav