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Preparation and characterization of heat stable and very active oxygen evolving photosystem ii particles from the thermophilic cyanobacterium synechococcus elongatus



Preparation and characterization of heat stable and very active oxygen evolving photosystem ii particles from the thermophilic cyanobacterium synechococcus elongatus



Plant & Cell Physiology 33(3): 299-305



Very active and heat-stable oxygen-evolving photosystem II particles were isolated from the thermophilic cyanobacterium Synechococcus elongatus by treatment of thylakoid membranes with a non-ionic detergent, sucrose monolaurate (SML). The particles were analyzed in a comparison with photosystem II particles prepared with .beta.-octylglucoside (OG). The two preparations had similar polypeptide compositions, which were characterized by high levels of polypeptides from phycobilisomes. The ratio of chlorophyll a to QA was 45 and there were four Mn atoms and one tightly bound CA2+ ion per QA in the particles prepared with SML. The preparations were thermophilic, showing substantial rates of oxygen evolution at temperatures up to 60.degree.C. The maximum rates attained at 45.degree.C were as high as 6.0 mmoles O2 mg-1 Chl h-1. PS II particles prepared with OG were similarly thermostable but were less active in oxygen evolution at all temperatures examined. Kinetic analysis of flash-induced absorption transients revealed that about 22% and 28% of photosystem II reaction centers were not associated with the functional QB site in the SML- and OG-particles, respectively. When corrected for the inactive reaction centers, the maximum rates of oxygen evolution by SML- and OG-particles were 7.7 and 7.0 mmoles O2 mg-1 Chl h-1, which correspond to half times of 1.9 and 2.1 ms for the first-order electron transfer, respectively. Comparison of these half times with those of the S-state transition and the release of oxygen indicates that the overall photosystem II electron transport is limited by the reduction of added electron acceptors and not by release of oxygen.

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