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Preparation and characterization of monoclonal antibodies directed against human respiratory syncytial virus subgroup b strain and antigenic analysis of subgroup b field strains with these monoclonal antibodies



Preparation and characterization of monoclonal antibodies directed against human respiratory syncytial virus subgroup b strain and antigenic analysis of subgroup b field strains with these monoclonal antibodies



Sapporo Medical Journal 59(3): 215-226



Twenty hybridomas producing monoclonal antibodies (MAbs) against the structural proteins of strain 58-17, a subgroup B strain of respiratory syncytial virus (RSV) were obtained by fusion of X63 myeloma cells with spleen cells from BALB/c mice immunized with virus=infected HEp-2 cells. Eight clines were found to produce antibodies against the fusion protein(F), five against the large glycoprotein (G), five against the nucleoprotein(NP) and two against the 24K protein by radioimmunoprecipitation assay(RIPA). By competitive binding assay with the MAbs, a minimum of eight, three, three and one epitopes were detected on the F, G, NP and 24 K protein components of subgroup B strain, respectively. Of these epitopes, the numbers of in subgroup A strain were 3/8, 2/3 and 1/3 on the F, G and NP components, respectively. The reactivities of 53 subgroup B field strains isolated in Sapporo, Japan, during nine epidemic years from 1980 to 1989, were examined with the MAbs using ELISA. Further differences of the reactivity to one anti-NP antibody enabled the classification of the B strains into 3 subgroups (B1 : 26 strains, B2 : 26 strains, and B3 : 1 strain). The dominant strain had changed from B1 to B2 strain during the 1985-1986 epidemic year. All 53 subgroup B strains reacted in a consistent manner with the other 19 MAbs representing 14 epitopes.

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