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Preparation and immunological properties of monoclonal antibodies to ribgrass mosaic virus rmvsh i. preparation of monoclonal antibodies subclasses determination and immunoprecipitin reaction


Preparation and immunological properties of monoclonal antibodies to ribgrass mosaic virus rmvsh i. preparation of monoclonal antibodies subclasses determination and immunoprecipitin reaction



Virologica Sinica 3(4): 364-369



ISSN/ISBN: 1674-0769

Using hybridoma techniques, seven hybridoma cell lines: 1H2, 7H1, 10H1, 11H2, 12H3, 17H6, 29H1, secreting monoclonal antibodies to Shanghai Isolate of Ribgrass Mosaic Virus (RMVsh) were prepared by fusion on myeloma cells SP2/O with spleen cells of BALB/c mouse immunized with RMVsh. The subclasses of these monoclonal antibodies were determined by double antibody sandwich ELISA. 1H2, 7H1 and other monoclonal antibodies belong to IgG3, IgG2b and IgG1 subclass, respectively. The ascitic fluids were obtained when these hybridoma cells were injected to BALB/c mice. A precipitin line was formed when monoclonal antibodies 1H2, 12H3 reacted with the antigen of RMVsh in agarose medium by double immunoprecipitin, but no precipitin line was formed with other five monoclonal antibodies. Monoclonal antibodies prepared here could be used in the diagnosis of infected plants and the analysis of the antigenic properties of the virus.

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Accession: 007680952

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