Primary structures of cytotoxic factors isolated from habu (Trimeresurus flavoviridis) venom
Yamakawa, Y.; Omori-Satoh, T.; Maeyama, J.
Journal of Biochemistry 109(5): 667-669
ISSN/ISBN: 0021-924X PMID: 1917887 Accession: 007687944
The amino acid sequence of a cytotoxic factor, CTF-I, isolated from the venom of the Japanese habu snake (Trimeresurus flavoviridis) has been determined through automatic phenylisothiocyanate degradation of the PE-protein and derived proteolytic peptides. CTF-I consists of 72 amino acids and contains an Arg-Gly-Asp sequence present in trigramin-like peptides isolated from other snake venoms. The primary structure of another cytotoxic factor, CTF-II, consisting of 75 amino acids, was deduced to comprise that of CTF-1 with an additional Glu-Leu-Leu-sequence at its N-terminal.