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Progress in cultural efficiency of rice anther culture for haploid breeding 1. reliable recommendable plant regeneration techniques for rice anther culture



Progress in cultural efficiency of rice anther culture for haploid breeding 1. reliable recommendable plant regeneration techniques for rice anther culture



Research Reports of the Rural Development Administration 31(1 BIOTECHNOL): 15-21



The present paper was proposed on recent progress in reliable, recommendable plant regeneration techniques for rice anther culture to obtain basic information for in vitro selection using haploid cell culture. Variation in culture efficiency was recognized according to pollen stage, degree of temperature and days treated as cold pre-treatment, modified sources and concentrations of nitrogen and plant growth regulators, and genotypic differences as donor plant. The salient findings obtained are summarized as follows; The best anther culturability was obtained when panicle development in leaf sheath reached up to auricles of 2nd leaf and anther development reached up to 1/3 of spikelet length for practical purpose. In the Tongil type rice cultivar Milyang 23, known to be reluctant genotype to anther culture, frequency of callus induction was 11.5% and green plant regeneration was 3.3%. The most effective culturability was obtained when panicles containing adequate pollen stage pretreated with 12.degree.C for 20 days. The frequency of green plant regeneration of 13.6% yielded in the Tongil type rice cultivar Milyang 23. Nitrogen sources and concentrations composed of 28 mM KNO3, 1.75 mM(NH4)2 SO4 and 1.75 mM L-Glutamine, although total nitrogen contents was the same of N6, yielded higher culturability than those of N6. When cold pretreatment applied, combination of 2.0 mg/l NAA, 1.0 mg/l kinetin, and 10.0 mg/l ABA showed the best response in culturability. The frequency of plant regeneration of 12.9% yielded in Japonica rice cultivar Nagdongbyeo. Combination with ABA was also effective maintaining of vitality of pollen-derived callus up to 70 days after anther inoculation. Genotypic difference in culturability was clearly recognized. The frequency of culturability of Japonica crosses have better response than those of Tongil or Indica.

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