+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Protoporphyrin IX photosensitization of corneal endothelium



Protoporphyrin IX photosensitization of corneal endothelium



Archives of Ophthalmology 107(10): 1497-1500



Protoporphyrin IX, a naturally occurring precursor of hemoglobin found in serum and erythrocytes, is a photoactive compound that we have detected in aqueous aspirates of three patients with hyphemas (0.13, 0.33, and 0.67 microgram/dL). To determine whether corneal endothelial photosensitization could occur, rabbit corneas were exposed to physiologic concentrations of protoporphyrin and light (200 microEinsteins [microE]/m2 per second). Experimental corneas demonstrated endothelial swelling with loss of intercellular contact by scanning electron microscopy. Flux chamber experiments demonstrated an increase in the net endothelial inflow, indicating a loss of the endothelial barrier function. Control corneas had normal ultrastructural architecture and flux study results. These studies suggest that patients with long-standing hyphemas who may be exposed to long periods of light are possibly at risk for developing endothelial dysfunction and corneal blood staining. Patching of these patients' affected eyes may be prudent.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 007702686

Download citation: RISBibTeXText

PMID: 2803100

DOI: 10.1001/archopht.1989.01070020571042


Related references

Photosensitization of rabbit corneal endothelium with protoporphyrin ix. Investigative Ophthalmology & Visual ScienceSuppl.: 171, 1987

Mechanism of phototoxicity of protoporphyrin and uroporphyrin on bovine corneal endothelium. Journal of Toxicology Cutaneous & Ocular Toxicology 13(1): 55-65, 1994

Sites of photosensitization by protoporphyrin and tin protoporphyrin in leukemia L1210 cells. Journal of Photochemistry and Photobiology. B Biology 6(1-2): 87-92, 1990

Reactive species involved in phototoxicity of protoporphyrin and uroporphyrin on bovine corneal endothelium. Free Radical Biology & Medicine 9(Suppl. 1): 75, 1990

Studies of the corneal endothelium by specular microscope. III. Effect of surgical procedures and the methods of corneal preservation on the endothelium. Klinika Oczna 87(4): 147-148, 1985

A method for registration of toxic drug effects on corneal endothelium. Effect of gentamicin on rabbit corneal endothelium. Acta Ophthalmologica 70(1): 101-107, 1992

Histochemical study of acid phosphatase activity in the normal corneal endothelium and the corneal endothelium after polystyrene latex particles injected into the anterior chamber in rabbit. Nippon Ganka Gakkai Zasshi 88(8): 1166-1175, 1984

Mechanisms of photosensitization by protoporphyrin coproporphyrin and uroporphyrin. Molecular Aspects of Medicine 11(1-2): 109, 1990

Photosensitization of retinal pigment epithelium by protoporphyrin IX. Graefe'S Archive For Clinical & Experimental Ophthalmology. 236(3): 230-233, Ch, 1998

Studies on the corneal and trabecular endothelium. I. Cement substance of the corneal endothelium. British Journal of Ophthalmology 42(9): 529-534, 1958

Photosensitization with endogenous protoporphyrin IX induces cell cycle changes. Photochemistry & Photobiology 67(SPEC ISSUE): 103S-104S, 1998

Photosensitization of red blood cell photohemolysis by protoporphyrin and other agents. Photochemistry & Photobiology 65(SPEC ISSUE): 7S, 1997

Reactive species involved in the photosensitization by protoporphyrin and uroporphyrin. Photochemistry & Photobiology 49(Suppl.): 48S, 1989

Physiological factors affecting ALA-induced protoporphyrin IX photosensitization in vitro. Photochemistry & Photobiology 61(5 Suppl. ): 94S, 1995