Purification of human 6-phosphogluconate dehydrogenase from human haemolysate with chromatography on an immobilized dye as the essential step and use of automation. Simultaneous purification of lactate dehydrogenase

Kroviarski, Y.; Cochet, S.; Vadon, C.; Truskolaski, A.; Boivin, P.; Bertrand, O.

Journal of Chromatography 449(2): 413-422

1988


ISSN/ISBN: 0021-9673
PMID: 3230114
Accession: 007709976

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Abstract
The screening procedure described in the preceding paper allowed a practical purification procedure to be devised that was automated for human 6-phosphogluconate dehydrogenase. The purification needed only two chromatographic steps, first on immobilized Procion Blue HE-GN and then on Phenyl-Sepharose. This technique also gave purified lactate dehydrogenase. Both enzymes showed single bands in SDS polyacrylamide gel electrophoresis.