EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Quantitative determination of beta exotoxin of bacillus thuringiensis in insecticide biopreparations


Biologicheskie Nauki (Moscow) (2): 119-126
Quantitative determination of beta exotoxin of bacillus thuringiensis in insecticide biopreparations
A method of quantitative determination of .beta.-exotoxin content in liquid and dry bioformulations has been developed. The method includes a thin-layer chromatography to isolate .beta.-exotoxin from accompanying nucleotides, the further desorption of a single .beta.-exotoxin spot by water and to carry our spectrophotometry at 259 and 330 nm. .beta.-exotoxin content in industrial formulations bitoxibacillin and turingin I has been determined. The results obtained correspond to the NMR1H spectroscopy data within the experimental errors. The relative error is 1-2%. The method sensitivity is 0.05 mg/ml. .beta.-exotoxin content at biotechnological stages of bitoxibacillin production has been determined.


Accession: 007714283



Related references

The quantitative determination of Bacillus thuringiensis beta-exotoxin in insecticidal biopreparations. Nauchnye Doklady Vysshei Shkoly. Biologicheskie Nauki: 119-126, 1990

Quantitative determination of beta -exotoxin of Bacillus thuringiensis in insecticidal biopreparations. Nauchnye Doklady Vysshei Shkoly, Biologicheskie Nauki ( 2): 119-126, 1990

Identification of beta-exotoxin production, plasmids encoding beta-exotoxin, and a new exotoxin in Bacillus thuringiensis by using high-performance liquid chromatography. Journal of Bacteriology 172(6): 3172-3179, 1990

Determination of beta-exotoxin in Iranian isolates of Bacillus thuringiensis. Journal of Entomological Society of Iran 23(1): 55-68, September, 2003

The efficacy of the delta endotoxin of bacillus thuringiensis ssp tenebrionis strain bi 256 82 in comparison with the beta exotoxin of bacillus thuringiensis ssp thuringiensis strain i 5 against first instar larvae of the colorado potato beetle leptinotarsa decemlineata. Mededelingen van de Faculteit Landbouwwetenschappen Universiteit Gent 53(3 PART A): 1017-1022, 1988

Quantitative analysis of beta-exotoxin in insecticidal preparations by high-performance anion-exchange chromatography Bacillus thuringiensis. Chemistry of natural compounds 18(3): 359-362, 1982

Larvicidal activity of the beta -exotoxin of Bacillus thuringiensis var. thuringiensis Berliner against two species of flies. Zeitschrift fur Angewandte Entomologie 74(2): 157-160, 1973

Sample preparation for beta-exotoxin determination in Bacillus thuringiensis cultures by reversed-phase high-performance liquid chromatography. Analytical Biochemistry 298(1): 112-117, 2001

Effectiveness of beta-exotoxin of Bacillus thuringiensis var. thuringiensis on the ability of Meloidogyne sp. from brinjal (Solanum melongenea L.) to survive. Journal of Biosciences 1(3): 271-278, 1979

Effect of the DD-136 nematode and of a beta -exotoxin preparation of Bacillus thuringiensis var. thuringiensis on leatherjackets, Tipula paludosa larvae. Journal of Invertebrate Pathology 20(2): 141-149, 1972