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Quantitative determination of beta exotoxin of bacillus thuringiensis in insecticide biopreparations


, : Quantitative determination of beta exotoxin of bacillus thuringiensis in insecticide biopreparations. Biologicheskie Nauki (Moscow) (2): 119-126

A method of quantitative determination of .beta.-exotoxin content in liquid and dry bioformulations has been developed. The method includes a thin-layer chromatography to isolate .beta.-exotoxin from accompanying nucleotides, the further desorption of a single .beta.-exotoxin spot by water and to carry our spectrophotometry at 259 and 330 nm. .beta.-exotoxin content in industrial formulations bitoxibacillin and turingin I has been determined. The results obtained correspond to the NMR1H spectroscopy data within the experimental errors. The relative error is 1-2%. The method sensitivity is 0.05 mg/ml. .beta.-exotoxin content at biotechnological stages of bitoxibacillin production has been determined.

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