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Radioimmunoassay of morphinan alkaloids in papaver somniferum hypocotyls callus and suspension cultures

Radioimmunoassay of morphinan alkaloids in papaver somniferum hypocotyls callus and suspension cultures

Journal of Plant Physiology 134(6): 645-650

Hypocotyls of poppy (Papaver somniferum L. cv. Marianne) were assayed for morphinan alkaloid content using a commercially available radioimmunoassay (RIA) for morphine (Abuscreen, Roche Diagnostics). The hypocotyls showed a sharp rise in morphinan alkaloid content on day 3 following sowing, reachin a plateau (9 ng .cntdot. mg-1 fresh wt) between day 4 and day 5. Hypocotyl explants were cultured for up to 2 months on solid nutrient medium containing .alpha.-naphthaleneacetic acid. During this time and while hypocotyls formed callus, the morphinan alkaloid content fell to a "maintenance" level of 0.4 ng .cntdot. mg-1 fresh weight in culture. Callus masses retained laticifier-like cells. If cell suspensions were plated with medium lacking growth regulators, plantlets were regenerated and the level of alkaloids found for hypocotyls was re-established. If plated cell suspensions were cultured in the presence of growth regulators, organs formed, but plantlets did not regenerate and the alkaloid content remained at or near the maintenance level. Over a period of a year callus subcultures lost their laticifer-like cells, their embryogenic potential, and their ability to maintain low-level alkaloid production.

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Accession: 007717811

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DOI: 10.1016/s0176-1617(89)80021-5

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