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Rapid high performance liquid chromatographic method for the analysis of debrisoquine and 4 hydroxydebrisoquine in urine without derivatization



Rapid high performance liquid chromatographic method for the analysis of debrisoquine and 4 hydroxydebrisoquine in urine without derivatization



Journal of Liquid Chromatography 13(5): 961-968



A simple and rapid HPLC method has been developed for the simultaneous determination of debrisoquine (D) and its main metabolite, 4-hydroxydebrisoquine (4OHD) in urine, without derivatization of the compounds. Guanoxan is used as the internal standard. The chromatographic separation was accomplished with a mobile phase comprising 15% acetonitrile and 85% 8 mM phosphate buffer (pH 5) at a flow rate of 1 ml/min. The HPLC column was packed with 5 .mu.m Spherisorb-CN. The eluant was monitored at 214 nm. The intra- and inter-assay coefficients of variation were less than 6%. The lowest limit of detection for D was 0.1 .mu.g/ml and for 4OHD was 0.05 .mu.g/ml. The method is suitable for determining the oxidation phenotype of populations and is presently being used to study the oxidation polymorphism in Maori and South Pacific Polynesians.

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Accession: 007720686

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